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10.1016/j.diagmicrobio.2020.115182 http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2020.115182 32947111!7441011!32947111     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Diagn+Microbiol+Infect+Dis 2020 ; 98 (4): 115182 Nephropedia Template TP {{tp|p=32947111|t=2020. Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature |pdf=|usr=}}{{32947111}} gab.com Text Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature JO: Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32947111 #FOAvip Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus causing a highly fatal respiratory disease in humans. Confirmation of MERS-CoV infection and molecular study on the virus may require transportation of samples to specialized laboratories. While freezing at -80? degrees C is the gold standard method for RNA preservation, maintaining the integrity of viral RNA during transport will require additional precautions and, as a result, increase transport costs. We aimed at testing the stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature for 16 weeks. Respiratory samples spiked with stock culture of MERS-CoV were extracted and loaded on QIAamp Viral RNA Mini Kit spin columns and preserved at room temperature. Amount of viral RNA was evaluated periodically by real-time quantitative reverse-transcription polymerase chain reaction. Minimal changes in cycle threshold values over the study period were noted, suggesting stability of viral RNA by this preservation method. Twit Text FOAVip Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature ????Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32947111 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32947111 #FOAvip English Wikipedia <ref>{{Cite pmid|32947111}}</ref> Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature #MMPMID32947111 Abdallah NMA; Zaki AM; Abdel-Salam SA Diagn Microbiol Infect Dis 2020[Dec]; 98 (4): 115182 PMID32947111show ga Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus causing a highly fatal respiratory disease in humans. Confirmation of MERS-CoV infection and molecular study on the virus may require transportation of samples to specialized laboratories. While freezing at -80? degrees C is the gold standard method for RNA preservation, maintaining the integrity of viral RNA during transport will require additional precautions and, as a result, increase transport costs. We aimed at testing the stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature for 16 weeks. Respiratory samples spiked with stock culture of MERS-CoV were extracted and loaded on QIAamp Viral RNA Mini Kit spin columns and preserved at room temperature. Amount of viral RNA was evaluated periodically by real-time quantitative reverse-transcription polymerase chain reaction. Minimal changes in cycle threshold values over the study period were noted, suggesting stability of viral RNA by this preservation method. |Coronavirus Infections/*diagnosis[MESH] |Humans[MESH] |Middle East Respiratory Syndrome Coronavirus/*genetics[MESH] |Mutation Rate[MESH] |Preservation, Biological/methods[MESH] |RNA Stability/*genetics[MESH] |RNA, Viral/*analysis[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH]Stability of MERS-CoV RNA on spin columns of RNA extraction kit at roo(epmc).pdf DeepDyve Pubget Overpricing