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10.1016/j.cca.2020.09.018

http://scihub22266oqcxt.onion/10.1016/j.cca.2020.09.018
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32946791!7492139!32946791
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suck abstract from ncbi


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pmid32946791      Clin+Chim+Acta 2020 ; 510 (ä): 717-722
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  • SARS-CoV-2 identification and IgA antibodies in saliva: One sample two tests approach for diagnosis #MMPMID32946791
  • Aita A; Basso D; Cattelan AM; Fioretto P; Navaglia F; Barbaro F; Stoppa A; Coccorullo E; Farella A; Socal A; Vettor R; Plebani M
  • Clin Chim Acta 2020[Nov]; 510 (ä): 717-722 PMID32946791show ga
  • AIM: This study aims to verify whether standardized saliva collection is suitable for SARS-CoV-2 molecular detection and IgA measurement. METHODS: 43 COVID-19 inpatients and 326 screening subjects underwent naso-pharyngeal (NP)-swab and saliva collection (Salivette). Inpatients also underwent repeated blood collections to evaluate inflammation and organs involvement. In all patients and subjects, SARS-CoV-2 (gene E) rRT-PCR was undertaken in saliva and NP-swabs. Salivary IgA and serum IgA, IgG, IgM were measured on inpatients' samples. RESULTS: NP-swabs and saliva were both SARS-CoV-2 positive in 7 (16%) or both negative in 35 (82%) out of 43 patients successfully included in the study. NP-swabs and saliva results did not perfectly match in one patient (saliva positive, NP-swab negative). Positive molecular results were significantly associated with disease duration (p = 0.0049). 326/326 screening subjects were SARS-CoV-2 negative on both NP-swabs and saliva. Among the 27 saliva samples tested for IgA, 18 were IgA positive. Salivary IgA positivity was associated with pneumonia (p = 0.002) and CRP values (p = 0.0183), not with other clinical and molecular data, or with serum immunoglubulins. CONCLUSIONS: A standardized saliva collection can be adopted to detect SARS-CoV-2 infection in alternative to NP-swabs. Preliminary data on salivary IgA support the use of saliva also for patient monitoring.
  • |*Clinical Laboratory Techniques[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Betacoronavirus/*immunology[MESH]
  • |COVID-19 Testing[MESH]
  • |Coronavirus Infections/diagnosis[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoglobulin A/*analysis/immunology[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Reference Standards[MESH]
  • |SARS-CoV-2[MESH]
  • |Saliva/*chemistry[MESH]


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