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10.1016/j.jviromet.2020.113972 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.113972 32941977!7490281!32941977     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2020 ; 286 (ä): 113972 Nephropedia Template TP {{tp|p=32941977|t=2020. Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2 |pdf=|usr=}}{{32941977}} gab.com Text Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2 JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32941977 #FOAvip A novel reverse-transcriptase loop mediated amplification (RT-LAMP) method targeting genes encoding the Spike (S) protein and RNA-dependent RNA polymerase (RdRP) of SARS-CoV-2 has been developed. The LAMP assay achieves a comparable limit of detection (25-50 copies per reaction) to commonly used RT-PCR protocols using clinical samples quantified by digital droplet PCR. Precision, cross-reactivity, inclusivity, and limit of detection studies were performed according to regulatory standards. Clinical validation of dual-target RT-LAMP (S and RdRP gene) achieved a PPA of 98.48 % (95 % CI 91.84%-99.96%) and NPA 100.00 % (95 % CI 93.84%-100.00%) based on the E gene and N2 gene reference RT-PCR methods. The method has implications for development of point of care technology using isothermal amplification. Twit Text FOAVip Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2 ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32941977 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32941977 #FOAvip English Wikipedia <ref>{{Cite pmid|32941977}}</ref> Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2 #MMPMID32941977 Mohon AN; Oberding L; Hundt J; van Marle G; Pabbaraju K; Berenger BM; Lisboa L; Griener T; Czub M; Doolan C; Servellita V; Chiu CY; Greninger AL; Jerome KR; Pillai DR J Virol Methods 2020[Dec]; 286 (ä): 113972 PMID32941977show ga A novel reverse-transcriptase loop mediated amplification (RT-LAMP) method targeting genes encoding the Spike (S) protein and RNA-dependent RNA polymerase (RdRP) of SARS-CoV-2 has been developed. The LAMP assay achieves a comparable limit of detection (25-50 copies per reaction) to commonly used RT-PCR protocols using clinical samples quantified by digital droplet PCR. Precision, cross-reactivity, inclusivity, and limit of detection studies were performed according to regulatory standards. Clinical validation of dual-target RT-LAMP (S and RdRP gene) achieved a PPA of 98.48 % (95 % CI 91.84%-99.96%) and NPA 100.00 % (95 % CI 93.84%-100.00%) based on the E gene and N2 gene reference RT-PCR methods. The method has implications for development of point of care technology using isothermal amplification. |Betacoronavirus/*genetics/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Computer Simulation[MESH] |Coronavirus Envelope Proteins[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/*virology[MESH] |Point-of-Care Systems[MESH] |RNA, Viral/genetics/*isolation & purification[MESH] |RNA-Directed DNA Polymerase[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Spike Glycoprotein, Coronavirus/genetics[MESH]Optimization and clinical validation of dual-target RT-LAMP for SARS-C(epmc).pdf DeepDyve Pubget Overpricing