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10.1080/22221751.2020.1825016

http://scihub22266oqcxt.onion/10.1080/22221751.2020.1825016
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suck abstract from ncbi


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pmid32940547      Emerg+Microbes+Infect 2020 ; 9 (1): 2157-2168
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  • Multicenter evaluation of two chemiluminescence and three lateral flow immunoassays for the diagnosis of COVID-19 and assessment of antibody dynamic responses to SARS-CoV-2 in Taiwan #MMPMID32940547
  • Chen SY; Lee YL; Lin YC; Lee NY; Liao CH; Hung YP; Lu MC; Wu JL; Tseng WP; Lin CH; Chung MY; Kang CM; Lee YF; Lee TF; Cheng CY; Chen CP; Huang CH; Liu CE; Cheng SH; Ko WC; Hsueh PR; Chen SC
  • Emerg Microbes Infect 2020[Dec]; 9 (1): 2157-2168 PMID32940547show ga
  • This multicenter, retrospective study included 346 serum samples from 74 patients with coronavirus disease 2019 (COVID-19) and 194 serum samples from non-COVID-19 patients to evaluate the performance of five anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody tests, i.e. two chemiluminescence immunoassays (CLIAs): Roche Elecsys(R) Anti-SARS-CoV-2 Test (Roche Test) and Abbott SARS-CoV-2 IgG (Abbott Test), and three lateral flow immunoassays (LFIAs): Wondfo SARS-CoV-2 Antibody Test (Wondfo Test), ASK COVID-19 IgG/IgM Rapid Test (ASK Test), and Dynamiker 2019-nCoV IgG/IgM Rapid Test (Dynamiker Test). We found high diagnostic sensitivities (%, 95% confidence interval [CI]) for the Roche Test (97.4%, 93.4-99.0%), Abbott Test (94.0%, 89.1-96.8%), Wondfo Test (91.4%, 85.8-94.9%), ASK Test (97.4%, 93.4-99.0%), and Dynamiker Test (90.1%, 84.3-94.0%) after >21 days of symptom onset. Meanwhile, the diagnostic specificity was 99.0% (95% CI, 96.3-99.7%) for the Roche Test, 97.9% (95% CI, 94.8-99.2%) for the Abbott Test, and 100.0% (95% CI, 98.1-100.0%) for the three LFIAs. Cross-reactivity was observed in sera containing anti-cytomegalovirus (CMV) IgG/IgM antibodies and autoantibodies. No difference was observed in the time to seroconversion detection of the five serological tests. Specimens from patients with COVID-19 pneumonia demonstrated a shorter seroconversion time and higher chemiluminescent signal than those without pneumonia. Our data suggested that understanding the dynamic antibody response after COVID-19 infection and performance characteristics of different serological test are crucial for the appropriate interpretation of serological test result for the diagnosis and risk assessment of patient with COVID-19 infection.
  • |Adult[MESH]
  • |Aged[MESH]
  • |Antibodies, Viral/blood/*immunology[MESH]
  • |Betacoronavirus/genetics/*immunology[MESH]
  • |COVID-19[MESH]
  • |Coronavirus Infections/*diagnosis/epidemiology/*immunology/virology[MESH]
  • |Cross Reactions/immunology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoassay/*methods/standards[MESH]
  • |Luminescent Measurements/*methods/standards[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/epidemiology/*immunology/virology[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2[MESH]
  • |Seroconversion[MESH]
  • |Serologic Tests[MESH]
  • |Severity of Illness Index[MESH]


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