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10.1172/jci.insight.142167 http://scihub22266oqcxt.onion/10.1172/jci.insight.142167 32937615!7605520!32937615     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       JCI+Insight 2020 ; 5 (20): ä Nephropedia Template TP {{tp|p=32937615|t=2020. High levels of SARS-CoV-2-specific T cells with restricted functionality in severe courses of COVID-19 |pdf=|usr=}}{{32937615}} gab.com Text High levels of SARS-CoV-2-specific T cells with restricted functionality in severe courses of COVID-19 JO: JCI Insight http://www.kidney.de/pget.php?&tw=1&pmid=32937615 #FOAvip BACKGROUNDPatients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) differ in the severity of disease. We hypothesized that characteristics of SARS-CoV-2-specific immunity correlate with disease severity.METHODSIn this study, SARS-CoV-2-specific T cells and antibodies were characterized in uninfected controls and patients with different coronavirus disease 2019 (COVID-19) disease severity. SARS-CoV-2-specific T cells were flow cytometrically quantified after stimulation with SARS-CoV-2 peptide pools and analyzed for expression of cytokines (IFN-gamma, IL-2, and TNF-alpha) and markers for activation, proliferation, and functional anergy. SARS-CoV-2-specific IgG and IgA antibodies were quantified using ELISA. Moreover, global characteristics of lymphocyte subpopulations were compared between patient groups and uninfected controls.RESULTSDespite severe lymphopenia affecting all major lymphocyte subpopulations, patients with severe disease mounted significantly higher levels of SARS-CoV-2-specific T cells as compared with convalescent individuals. SARS-CoV-2-specific CD4+ T cells dominated over CD8+ T cells and closely correlated with the number of plasmablasts and SARS-CoV-2-specific IgA and IgG levels. Unlike in convalescent patients, SARS-CoV-2-specific T cells in patients with severe disease showed marked alterations in phenotypical and functional properties, which also extended to CD4+ and CD8+ T cells in general.CONCLUSIONGiven the strong induction of specific immunity to control viral replication in patients with severe disease, the functionally altered characteristics may result from the need for contraction of specific and general immunity to counteract excessive immunopathology in the lung.FUNDINGThe study was supported by institutional funds to MS and in part by grants of Saarland University, the State of Saarland, and the Rolf M. Schwiete Stiftung. Twit Text FOAVip High levels of SARS-CoV-2-specific T cells with restricted functionality in severe courses of COVID-19 ????JCI Insight http://www.kidney.de/pget.php?&tw=1&pmid=32937615 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32937615 #FOAvip English Wikipedia <ref>{{Cite pmid|32937615}}</ref> High levels of SARS-CoV-2-specific T cells with restricted functionality in severe courses of COVID-19 #MMPMID32937615 Schub D; Klemis V; Schneitler S; Mihm J; Lepper PM; Wilkens H; Bals R; Eichler H; Gartner BC; Becker SL; Sester U; Sester M; Schmidt T JCI Insight 2020[Oct]; 5 (20): ä PMID32937615show ga BACKGROUNDPatients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) differ in the severity of disease. We hypothesized that characteristics of SARS-CoV-2-specific immunity correlate with disease severity.METHODSIn this study, SARS-CoV-2-specific T cells and antibodies were characterized in uninfected controls and patients with different coronavirus disease 2019 (COVID-19) disease severity. SARS-CoV-2-specific T cells were flow cytometrically quantified after stimulation with SARS-CoV-2 peptide pools and analyzed for expression of cytokines (IFN-gamma, IL-2, and TNF-alpha) and markers for activation, proliferation, and functional anergy. SARS-CoV-2-specific IgG and IgA antibodies were quantified using ELISA. Moreover, global characteristics of lymphocyte subpopulations were compared between patient groups and uninfected controls.RESULTSDespite severe lymphopenia affecting all major lymphocyte subpopulations, patients with severe disease mounted significantly higher levels of SARS-CoV-2-specific T cells as compared with convalescent individuals. SARS-CoV-2-specific CD4+ T cells dominated over CD8+ T cells and closely correlated with the number of plasmablasts and SARS-CoV-2-specific IgA and IgG levels. Unlike in convalescent patients, SARS-CoV-2-specific T cells in patients with severe disease showed marked alterations in phenotypical and functional properties, which also extended to CD4+ and CD8+ T cells in general.CONCLUSIONGiven the strong induction of specific immunity to control viral replication in patients with severe disease, the functionally altered characteristics may result from the need for contraction of specific and general immunity to counteract excessive immunopathology in the lung.FUNDINGThe study was supported by institutional funds to MS and in part by grants of Saarland University, the State of Saarland, and the Rolf M. Schwiete Stiftung. |*Antibodies, Viral/blood/classification[MESH] |*Betacoronavirus/immunology/isolation & purification[MESH] |*Coronavirus Infections/blood/epidemiology/physiopathology/virology[MESH] |*Leukocyte Count/methods/statistics & numerical data[MESH] |*Pandemics[MESH] |*Pneumonia, Viral/blood/epidemiology/physiopathology/virology[MESH] |*T-Lymphocytes/classification/virology[MESH] |Adult[MESH] |COVID-19[MESH] |Cardiovascular Diseases/epidemiology[MESH] |Comorbidity[MESH] |Correlation of Data[MESH] |Critical Care/methods/statistics & numerical data[MESH] |Critical Illness/therapy[MESH] |Cytokines/*blood[MESH] |Female[MESH] |Germany/epidemiology[MESH] |Humans[MESH] |Lymphocyte Subsets/classification[MESH] |Male[MESH] |Metabolic Diseases/epidemiology[MESH] |Middle Aged[MESH] |SARS-CoV-2[MESH]High levels of SARS-CoV-2-specific T cells with restricted functionali(epmc).pdf DeepDyve Pubget Overpricing