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10.1016/j.jcv.2020.104632

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suck abstract from ncbi


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pmid32932152      J+Clin+Virol 2020 ; 132 (ä): 104632
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  • Comparison of the GeneFinder(TM) COVID-19 Plus RealAmp Kit on the sample-to-result Platform ELITe InGenius to the national reference method: An added value of N gene target detection? #MMPMID32932152
  • Ong DSY; Claas ECJ; Breijer S; Vaessen N
  • J Clin Virol 2020[Nov]; 132 (ä): 104632 PMID32932152show ga
  • BACKGROUND: Due to the emergence of the coronavirus disease 2019 (COVID-19) pandemic there is an urgent need for rapid and accurate testing on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). OBJECTIVES: The aim of this study was to assess the diagnostic performance of the GeneFinder(TM)COVID-19 Plus RealAmp Kit on the ELITe InGenius sample-to-result platform, which is a commercial nucleic acid amplification test (NAT) targeting genes of SARS-CoV-2. STUDY DESIGN: Patients were eligible between March 18 and May 27, 2020, when they had respiratory symptoms that were suspected for COVID-19. The InGenius platform was compared to routine in-house NAT that was validated according to the national reference. RESULTS: Of 128 randomly selected patients, 58 (45 %) tested positive and 55 (43 %) tested negative in both platforms. Sensitivity of the InGenius platform was 100 % (95 % confidence interval 94-100). In the remaining 15 (12 %) cases E and RdRp genes were not detected in both platforms but the nucleoprotein (N) gene was tested positive by the InGenius platform. All solitary N gene positive cases were confirmed by a N-gene specific in-house validated NAT, and most of these patients could also be considered positive based on other recently available COVID-19 positive respiratory samples or highly suspected radiological findings. CONCLUSION: The InGenius platform for SARS-CoV-2 detection has excellent sensitivity, is easy to use and provides fast results. The inclusion of the N gene as a third gene target may further increase sensitivity for the diagnosis of COVID-19 in comparison to the national reference method.
  • |*COVID-19 Testing/methods/standards[MESH]
  • |*Nucleic Acid Amplification Techniques/methods/standards[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Molecular Diagnostic Techniques[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2/*genetics[MESH]


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