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10.1016/j.jcv.2020.104618

http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104618
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32919222!7470702!32919222
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suck abstract from ncbi


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pmid32919222      J+Clin+Virol 2020 ; 132 (ä): 104618
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  • Performance evaluation of two SARS-CoV-2 IgG/IgM rapid tests (Covid-Presto and NG-Test) and one IgG automated immunoassay (Abbott) #MMPMID32919222
  • Charpentier C; Ichou H; Damond F; Bouvet E; Chaix ML; Ferre V; Delaugerre C; Mahjoub N; Larrouy L; Le Hingrat Q; Visseaux B; Mackiewicz V; Descamps D; Fidouh-Houhou N
  • J Clin Virol 2020[Nov]; 132 (ä): 104618 PMID32919222show ga
  • The aim of this study was to assess the analytical performances, sensitivity and specificity, of two rapid tests (Covid- Presto(R) test rapid Covid-19 IgG/IgM and NG-Test(R) IgM-IgG COVID-19) and one automated immunoassay (Abbott SARS-CoV-2 IgG) for detecting anti- SARS-CoV-2 antibodies. This study was performed with: (i) a positive panel constituted of 88 SARS-CoV-2 specimens collected from patients with a positive SARS-CoV-2 RT-PCR, and (ii) a negative panel of 120 serum samples, all collected before November 2019, including 64 samples with a cross-reactivity panel. Sensitivity of Covid-Presto(R) test for IgM and IgG was 78.4% and 92.0%, respectively. Sensitivity of NG-Test(R) for IgM and IgG was 96.6% and 94.9%, respectively. Sensitivity of Abbott IgG assay was 96.5% showing an excellent agreement with the two rapid tests (kappa = 0.947 and kappa = 0.936 for NGTest (R) and Covid-Presto(R) test, respectively). An excellent agreement was also observed between the two rapid tests (kappa = 0.937). Specificity for IgM was 100% and 86.5% for Covid-Presto(R) test and NG-Test(R), respectively. Specificity for IgG was 92.0%, 94.9% and 96.5% for Covid-Presto(R), NGTest (R), and Abbott, respectively. Most of the false positive results observed with NG-Test(R) resulted from samples containing malarial antibodies. In conclusion, performances of these 2 rapid tests are very good and comparable to those obtained with automated immunoassay, except for IgM specificity with the NG-Test(R). Thus, isolated IgM should be cautiously interpreted due to the possible false-positive reactions with this test. Finally, before their large use, the rapid tests must be reliably evaluated with adequate and large panel including early seroconversion and possible cross-reactive samples.
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |COVID-19 Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoassay/*methods[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Immunoglobulin M/blood[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |SARS-CoV-2/*immunology[MESH]


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