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10.1016/j.jviromet.2020.113969 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.113969 32918932!7480275!32918932     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32918932&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 287 (ä): 113969 Nephropedia Template TP {{tp|p=32918932|t=2021. Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction |pdf=|usr=}}{{32918932}} gab.com Text Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32918932 #FOAvip The current COVID-19 pandemic constitutes a threat to the population worldwide with over 21 million infected people. There is an urgent need for the development of rapid and massive detection tools as well as the identification and isolation of infected individuals. we sought to evaluate different RT-qPCR kits and protocols to evaluate the best approach to be used omitting an RNA extraction step. We have investigated the sensitivity and performance of different commercially available RT-qPCR kits in detecting SARS-CoV-2 using 80 extracted RNA and NSS from COVID-19 diagnosed patients. We evaluated the ability of each kit to detect viral RNA from both kit-extracted or directly from a pre-boiled NSS observing that direct RNA detection is possible when Ct values are lower than 30 with the three kits tested. Since SARS-CoV-2 testing in most locations occurs once COVID-19 symptoms are evident and, therefore, viral loads are expected to be high, our protocol will be useful in supporting SARS-CoV-2 diagnosis, especially in America where COVID-19 cases have exploded in the recent weeks as well as in low- and middle-income countries, which would not have massive access to kit-based diagnosis. The information provided in this work paves the way for the development of more efficient SARS-CoV-2 detection approaches avoiding an RNA extraction step. Twit Text FOAVip Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32918932 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32918932 #FOAvip English Wikipedia <ref>{{Cite pmid|32918932}}</ref> Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction #MMPMID32918932 Beltran-Pavez C; Alonso-Palomares LA; Valiente-Echeverria F; Gaggero A; Soto-Rifo R; Barriga GP J Virol Methods 2021[Jan]; 287 (ä): 113969 PMID32918932show ga The current COVID-19 pandemic constitutes a threat to the population worldwide with over 21 million infected people. There is an urgent need for the development of rapid and massive detection tools as well as the identification and isolation of infected individuals. we sought to evaluate different RT-qPCR kits and protocols to evaluate the best approach to be used omitting an RNA extraction step. We have investigated the sensitivity and performance of different commercially available RT-qPCR kits in detecting SARS-CoV-2 using 80 extracted RNA and NSS from COVID-19 diagnosed patients. We evaluated the ability of each kit to detect viral RNA from both kit-extracted or directly from a pre-boiled NSS observing that direct RNA detection is possible when Ct values are lower than 30 with the three kits tested. Since SARS-CoV-2 testing in most locations occurs once COVID-19 symptoms are evident and, therefore, viral loads are expected to be high, our protocol will be useful in supporting SARS-CoV-2 diagnosis, especially in America where COVID-19 cases have exploded in the recent weeks as well as in low- and middle-income countries, which would not have massive access to kit-based diagnosis. The information provided in this work paves the way for the development of more efficient SARS-CoV-2 detection approaches avoiding an RNA extraction step. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |RNA, Viral/genetics/isolation & purification[MESH] |Reagent Kits, Diagnostic[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH] |Sensitivity and Specificity[MESH] |Specimen Handling/*methods[MESH] |Time Factors[MESH]Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA ext(epmc).pdf DeepDyve Pubget Overpricing