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10.1186/s12874-020-01110-y

http://scihub22266oqcxt.onion/10.1186/s12874-020-01110-y
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32917141!7483494!32917141
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suck abstract from ncbi


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pmid32917141      BMC+Med+Res+Methodol 2020 ; 20 (1): 228
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  • Establishment of a pediatric COVID-19 biorepository: unique considerations and opportunities for studying the impact of the COVID-19 pandemic on children #MMPMID32917141
  • Lima R; Gootkind EF; De la Flor D; Yockey LJ; Bordt EA; D'Avino P; Ning S; Heath K; Harding K; Zois J; Park G; Hardcastle M; Grinke KA; Grimmel S; Davidson SP; Forde PJ; Hall KE; Neilan AM; Matute JD; Lerou PH; Fasano A; Shui JE; Edlow AG; Yonker LM
  • BMC Med Res Methodol 2020[Sep]; 20 (1): 228 PMID32917141show ga
  • BACKGROUND: COVID-19, the disease caused by the highly infectious and transmissible coronavirus SARS-CoV-2, has quickly become a morbid global pandemic. Although the impact of SARS-CoV-2 infection in children is less clinically apparent, collecting high-quality biospecimens from infants, children, and adolescents in a standardized manner during the COVID-19 pandemic is essential to establish a biologic understanding of the disease in the pediatric population. This biorepository enables pediatric centers world-wide to collect samples uniformly to drive forward our understanding of COVID-19 by addressing specific pediatric and neonatal COVID-19-related questions. METHODS: A COVID-19 biospecimen collection study was implemented with strategic enrollment guidelines to include patients seen in urgent care clinics and hospital settings, neonates born to SARS-CoV-2 infected mothers, and asymptomatic children. The methodology described here, details the importance of establishing collaborations between the clinical and research teams to harmonize protocols for patient recruitment and sample collection, processing and storage. It also details modifications required for biobanking during a surge of the COVID-19 pandemic. RESULTS: Considerations and challenges facing enrollment of neonatal and pediatric cohorts are described. A roadmap is laid out for successful collection, processing, storage and database management of multiple pediatric samples such as blood, nasopharyngeal and oropharyngeal swabs, sputum, saliva, tracheal aspirates, stool, and urine. Using this methodology, we enrolled 327 participants, who provided a total of 972 biospecimens. CONCLUSIONS: Pediatric biospecimens will be key in answering questions relating to viral transmission by children, differences between pediatric and adult viral susceptibility and immune responses, the impact of maternal SARS-CoV-2 infection on fetal development, and factors driving the Multisystem Inflammatory Syndrome in Children. The specimens in this biorepository will allow necessary comparative studies between children and adults, help determine the accuracy of current pediatric viral testing techniques, in addition to, understanding neonatal exposure to SARS-CoV-2 infection and disease abnormalities. The successful establishment of a pediatric biorepository is critical to provide insight into disease pathogenesis, and subsequently, develop future treatment and vaccination strategies.
  • |*Betacoronavirus[MESH]
  • |Adolescent[MESH]
  • |COVID-19[MESH]
  • |Child[MESH]
  • |Child, Preschool[MESH]
  • |Coronavirus Infections/*diagnosis/immunology/transmission[MESH]
  • |Female[MESH]
  • |Fetal Development[MESH]
  • |Hospitalization[MESH]
  • |Humans[MESH]
  • |Infant[MESH]
  • |Infant, Newborn[MESH]
  • |Male[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/immunology/transmission[MESH]
  • |SARS-CoV-2[MESH]


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