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10.3390/v12091004 http://scihub22266oqcxt.onion/10.3390/v12091004 32911874!7551632!32911874     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2020 ; 12 (9): ä Nephropedia Template TP {{tp|p=32911874|t=2020. Infection of Brain Organoids and 2D Cortical Neurons with SARS-CoV-2 Pseudovirus |pdf=|usr=}}{{32911874}} gab.com Text Infection of Brain Organoids and 2D Cortical Neurons with SARS-CoV-2 Pseudovirus JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32911874 #FOAvip Since the global outbreak of SARS-CoV-2 (COVID-19), infections of diverse human organs along with multiple symptoms continue to be reported. However, the susceptibility of the brain to SARS-CoV-2, and the mechanisms underlying neurological infection are still elusive. Here, we utilized human embryonic stem cell-derived brain organoids and monolayer cortical neurons to investigate infection of brain with pseudotyped SARS-CoV-2 viral particles. Spike-containing SARS-CoV-2 pseudovirus infected neural layers within brain organoids. The expression of ACE2, a host cell receptor for SARS-CoV-2, was sustained during the development of brain organoids, especially in the somas of mature neurons, while remaining rare in neural stem cells. However, pseudotyped SARS-CoV-2 was observed in the axon of neurons, which lack ACE2. Neural infectivity of SARS-CoV-2 pseudovirus did not increase in proportion to viral load, but only 10% of neurons were infected. Our findings demonstrate that brain organoids provide a useful model for investigating SARS-CoV-2 entry into the human brain and elucidating the susceptibility of the brain to SARS-CoV-2. Twit Text FOAVip Infection of Brain Organoids and 2D Cortical Neurons with SARS-CoV-2 Pseudovirus ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32911874 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32911874 #FOAvip English Wikipedia <ref>{{Cite pmid|32911874}}</ref> Infection of Brain Organoids and 2D Cortical Neurons with SARS-CoV-2 Pseudovirus #MMPMID32911874 Yi SA; Nam KH; Yun J; Gim D; Joe D; Kim YH; Kim HJ; Han JW; Lee J Viruses 2020[Sep]; 12 (9): ä PMID32911874show ga Since the global outbreak of SARS-CoV-2 (COVID-19), infections of diverse human organs along with multiple symptoms continue to be reported. However, the susceptibility of the brain to SARS-CoV-2, and the mechanisms underlying neurological infection are still elusive. Here, we utilized human embryonic stem cell-derived brain organoids and monolayer cortical neurons to investigate infection of brain with pseudotyped SARS-CoV-2 viral particles. Spike-containing SARS-CoV-2 pseudovirus infected neural layers within brain organoids. The expression of ACE2, a host cell receptor for SARS-CoV-2, was sustained during the development of brain organoids, especially in the somas of mature neurons, while remaining rare in neural stem cells. However, pseudotyped SARS-CoV-2 was observed in the axon of neurons, which lack ACE2. Neural infectivity of SARS-CoV-2 pseudovirus did not increase in proportion to viral load, but only 10% of neurons were infected. Our findings demonstrate that brain organoids provide a useful model for investigating SARS-CoV-2 entry into the human brain and elucidating the susceptibility of the brain to SARS-CoV-2. |Angiotensin-Converting Enzyme 2[MESH] |Axons/enzymology[MESH] |Betacoronavirus/*physiology[MESH] |Cell Differentiation[MESH] |Cells, Cultured[MESH] |Cerebral Cortex/cytology[MESH] |Embryonic Stem Cells/virology[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Nerve Tissue Proteins/physiology[MESH] |Neural Stem Cells/enzymology/virology[MESH] |Neurons/enzymology/*virology[MESH] |Organoids/*virology[MESH] |Peptidyl-Dipeptidase A/physiology[MESH] |Prosencephalon/cytology/*virology[MESH] |Receptors, Virus/physiology[MESH] |SARS-CoV-2[MESH] |Spike Glycoprotein, Coronavirus/*physiology[MESH] |Viral Load[MESH] |Viral Tropism[MESH]Infection of Brain Organoids and 2D Cortical Neurons with SARS-CoV-2 P(epmc).pdf DeepDyve Pubget Overpricing