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10.1016/j.bios.2020.112578 http://scihub22266oqcxt.onion/10.1016/j.bios.2020.112578 32911317!7467868!32911317     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2020 ; 169 (ä): 112578 Nephropedia Template TP {{tp|p=32911317|t=2020. Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip |pdf=|usr=}}{{32911317}} gab.com Text Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=32911317 #FOAvip The ongoing global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to active research in its associated diagnostics and medical treatments. While quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most reliable method to detect viral genes of SARS-CoV-2, serological tests for specific antiviral antibodies are also important as they identify false negative qRT-PCR responses, track how effectively the patient's immune system is fighting the infection, and are potentially helpful for plasma transfusion therapies. In this work, based on the principle of localized surface plasmon resonance (LSPR), we develop an opto-microfluidic sensing platform with gold nanospikes, fabricated by electrodeposition, to detect the presence and amount of antibodies specific to the SARS-CoV-2 spike protein in 1muL of human plasma diluted in 1mL of buffer solution, within approximately 30min. The target antibody concentration can be correlated with the LSPR wavelength peak shift of gold nanospikes caused by the local refractive index change due to the antigen-antibody binding. This label-free microfluidic platform achieves a limit of detection of approximately 0.08ng/mL ( approximately 0.5pM), falling under the clinical relevant concentration range. We demonstrate that our opto-microfluidic platform offers a promising point-of-care testing tool to complement standard serological assays and make SARS-CoV-2 quantitative diagnostics easier, cheaper, and faster. Twit Text FOAVip Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=32911317 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32911317 #FOAvip English Wikipedia <ref>{{Cite pmid|32911317}}</ref> Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip #MMPMID32911317 Funari R; Chu KY; Shen AQ Biosens Bioelectron 2020[Dec]; 169 (ä): 112578 PMID32911317show ga The ongoing global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to active research in its associated diagnostics and medical treatments. While quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most reliable method to detect viral genes of SARS-CoV-2, serological tests for specific antiviral antibodies are also important as they identify false negative qRT-PCR responses, track how effectively the patient's immune system is fighting the infection, and are potentially helpful for plasma transfusion therapies. In this work, based on the principle of localized surface plasmon resonance (LSPR), we develop an opto-microfluidic sensing platform with gold nanospikes, fabricated by electrodeposition, to detect the presence and amount of antibodies specific to the SARS-CoV-2 spike protein in 1muL of human plasma diluted in 1mL of buffer solution, within approximately 30min. The target antibody concentration can be correlated with the LSPR wavelength peak shift of gold nanospikes caused by the local refractive index change due to the antigen-antibody binding. This label-free microfluidic platform achieves a limit of detection of approximately 0.08ng/mL ( approximately 0.5pM), falling under the clinical relevant concentration range. We demonstrate that our opto-microfluidic platform offers a promising point-of-care testing tool to complement standard serological assays and make SARS-CoV-2 quantitative diagnostics easier, cheaper, and faster. |Antibodies, Viral/*blood/immunology[MESH] |Betacoronavirus/*immunology[MESH] |COVID-19[MESH] |Coronavirus Infections/*blood/immunology/virology[MESH] |Equipment Design[MESH] |Gold/chemistry[MESH] |Humans[MESH] |Lab-On-A-Chip Devices[MESH] |Limit of Detection[MESH] |Nanostructures/*chemistry/ultrastructure[MESH] |Pandemics[MESH] |Pneumonia, Viral/*blood/immunology/virology[MESH] |SARS-CoV-2[MESH] |Spike Glycoprotein, Coronavirus/*immunology[MESH]Detection of antibodies against SARS-CoV-2 spike protein by gold nanos(epmc).pdf DeepDyve Pubget Overpricing