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10.1016/j.cca.2020.09.005 http://scihub22266oqcxt.onion/10.1016/j.cca.2020.09.005 32910979!7476884!32910979     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Chim+Acta 2020 ; 510 (ä): 760-766 Nephropedia Template TP {{tp|p=32910979|t=2020. Performance of an automated chemiluminescence SARS-CoV-2 IG-G assay |pdf=|usr=}}{{32910979}} gab.com Text Performance of an automated chemiluminescence SARS-CoV-2 IG-G assay JO: Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=32910979 #FOAvip INTRODUCTION: We describe our evaluation of the Abbott SARS-CoV-2 IgG assay on the Architect immunoassay analyser. METHODS: We assessed assay precision, sensitivity, specificity, positive/negative predictive values (PPV/NPV), cross-reactivity (influenza/dengue/hepatitis B and C/rheumatoid factor/anti-nuclear/double-stranded DNA/syphilis) and sample throughput in samples from real-time polymerase chain reaction (RT-PCR) positive patients/healthcare workers (HCWs)/pre-pandemic samples. We compared the cut-off indexes (COIs) between all control samples (HCWs and pre-pandemic) to generate an optimised COI limit for reactivity. RESULTS: The assay specificity was 99.8% (n = 980) and sensitivity was 45.9-96.7% (n = 279). When tested >/= 14 days post-positive RT-PCR (POS), the PPV/NPV was 96.4%/99.8%. The difference between the COIs of HCWs/pre-pandemic samples was small (0.01, p < 0.0001). There was minimal cross-reactivity with other antibodies. A lower COI limit for reactivity (>/=0.55, using the 99th percentile COI of our controls and ROC analysis) improved diagnostic sensitivity, especially at 0-6 days POS (45.9-55.8%), with a small decrease in specificity (98.9%). The assay throughput was 100 samples in 70 min. CONCLUSION: The Abbott SARS-CoV-2 IgG assay shows excellent performance in patients >/= 14 days POS. The difference between the COIs of HCWs and pre-pandemic samples was numerically small. A lower COI limit improves assay sensitivity with a slight decrease in specificity. Twit Text FOAVip Performance of an automated chemiluminescence SARS-CoV-2 IG-G assay ????Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=32910979 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32910979 #FOAvip English Wikipedia <ref>{{Cite pmid|32910979}}</ref> Performance of an automated chemiluminescence SARS-CoV-2 IG-G assay #MMPMID32910979 Lau CS; Oh HML; Hoo SP; Liang YL; Phua SK; Aw TC Clin Chim Acta 2020[Nov]; 510 (ä): 760-766 PMID32910979show ga INTRODUCTION: We describe our evaluation of the Abbott SARS-CoV-2 IgG assay on the Architect immunoassay analyser. METHODS: We assessed assay precision, sensitivity, specificity, positive/negative predictive values (PPV/NPV), cross-reactivity (influenza/dengue/hepatitis B and C/rheumatoid factor/anti-nuclear/double-stranded DNA/syphilis) and sample throughput in samples from real-time polymerase chain reaction (RT-PCR) positive patients/healthcare workers (HCWs)/pre-pandemic samples. We compared the cut-off indexes (COIs) between all control samples (HCWs and pre-pandemic) to generate an optimised COI limit for reactivity. RESULTS: The assay specificity was 99.8% (n = 980) and sensitivity was 45.9-96.7% (n = 279). When tested >/= 14 days post-positive RT-PCR (POS), the PPV/NPV was 96.4%/99.8%. The difference between the COIs of HCWs/pre-pandemic samples was small (0.01, p < 0.0001). There was minimal cross-reactivity with other antibodies. A lower COI limit for reactivity (>/=0.55, using the 99th percentile COI of our controls and ROC analysis) improved diagnostic sensitivity, especially at 0-6 days POS (45.9-55.8%), with a small decrease in specificity (98.9%). The assay throughput was 100 samples in 70 min. CONCLUSION: The Abbott SARS-CoV-2 IgG assay shows excellent performance in patients >/= 14 days POS. The difference between the COIs of HCWs and pre-pandemic samples was numerically small. A lower COI limit improves assay sensitivity with a slight decrease in specificity. |Adult[MESH] |Automation[MESH] |Betacoronavirus/*immunology[MESH] |Cross Reactions[MESH] |Female[MESH] |Humans[MESH] |Immunoglobulin G/*analysis/immunology[MESH] |Limit of Detection[MESH] |Luminescent Measurements/*methods[MESH] |Male[MESH] |Middle Aged[MESH] |Predictive Value of Tests[MESH]Performance of an automated chemiluminescence SARS-CoV-2 IG-G assay (epmc).pdf DeepDyve Pubget Overpricing