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Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Eur+J+Immunol 2020 ; 50 (9): 1283-1294 Nephropedia Template TP
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Expansion of plasmablasts and loss of memory B cells in peripheral blood from COVID-19 patients with pneumonia #MMPMID32910469
De Biasi S; Lo Tartaro D; Meschiari M; Gibellini L; Bellinazzi C; Borella R; Fidanza L; Mattioli M; Paolini A; Gozzi L; Jaacoub D; Faltoni M; Volpi S; Milic J; Sita M; Sarti M; Pucillo C; Girardis M; Guaraldi G; Mussini C; Cossarizza A
Eur J Immunol 2020[Sep]; 50 (9): 1283-1294 PMID32910469show ga
Studies on the interactions between SARS-CoV-2 and humoral immunity are fundamental to elaborate effective therapies including vaccines. We used polychromatic flow cytometry, coupled with unsupervised data analysis and principal component analysis (PCA), to interrogate B cells in untreated patients with COVID-19 pneumonia. COVID-19 patients displayed normal plasma levels of the main immunoglobulin classes, of antibodies against common antigens or against antigens present in common vaccines. However, we found a decreased number of total and naive B cells, along with decreased percentages and numbers of memory switched and unswitched B cells. On the contrary, IgM(+) and IgM(-) plasmablasts were significantly increased. In vitro cell activation revealed that B lymphocytes showed a normal proliferation index and number of dividing cells per cycle. PCA indicated that B-cell number, naive and memory B cells but not plasmablasts clustered with patients who were discharged, while plasma IgM level, C-reactive protein, D-dimer, and SOFA score with those who died. In patients with pneumonia, the derangement of the B-cell compartment could be one of the causes of the immunological failure to control SARS-Cov2, have a relevant influence on several pathways, organs and systems, and must be considered to develop vaccine strategies.