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10.1016/j.xcrm.2020.100099

http://scihub22266oqcxt.onion/10.1016/j.xcrm.2020.100099
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32905045!7462534!32905045
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suck abstract from ncbi


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pmid32905045      Cell+Rep+Med 2020 ; 1 (6): 100099
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  • Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant #MMPMID32905045
  • Mlcochova P; Collier D; Ritchie A; Assennato SM; Hosmillo M; Goel N; Meng B; Chatterjee K; Mendoza V; Temperton N; Kiss L; James LC; Ciazynska KA; Xiong X; Briggs JAG; Nathan JA; Mescia F; Bergamaschi L; Zhang H; Barmpounakis P; Demeris N; Skells R; Lyons PA; Bradley J; Baker S; Allain JP; Smith KGC; Bousfield R; Wilson M; Sparkes D; Amoroso G; Gkrania-Klotsas E; Hardwick S; Boyle A; Goodfellow I; Gupta RK
  • Cell Rep Med 2020[Sep]; 1 (6): 100099 PMID32905045show ga
  • Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%-50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8-92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity.
  • |*Point-of-Care Testing[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Antibodies, Viral/blood[MESH]
  • |COVID-19 Testing/*methods/standards[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Immunoassay[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Neutralization Tests[MESH]
  • |Nucleic Acid Amplification Techniques[MESH]
  • |SARS-CoV-2/genetics/immunology/*isolation & purification[MESH]
  • |Sensitivity and Specificity[MESH]


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