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10.1038/s41467-020-18130-3 http://scihub22266oqcxt.onion/10.1038/s41467-020-18130-3 32900994!7479142!32900994     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2020 ; 11 (1): 4464 Nephropedia Template TP {{tp|p=32900994|t=2020. A role for Biofoundries in rapid development and validation of automated SARS-CoV-2 clinical diagnostics |pdf=|usr=}}{{32900994}} gab.com Text A role for Biofoundries in rapid development and validation of automated SARS-CoV-2 clinical diagnostics JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=32900994 #FOAvip The SARS-CoV-2 pandemic has shown how a rapid rise in demand for patient and community sample testing can quickly overwhelm testing capability globally. With most diagnostic infrastructure dependent on specialized instruments, their exclusive reagent supplies quickly become bottlenecks, creating an urgent need for approaches to boost testing capacity. We address this challenge by refocusing the London Biofoundry onto the development of alternative testing pipelines. Here, we present a reagent-agnostic automated SARS-CoV-2 testing platform that can be quickly deployed and scaled. Using an in-house-generated, open-source, MS2-virus-like particle (VLP) SARS-CoV-2 standard, we validate RNA extraction and RT-qPCR workflows as well as two detection assays based on CRISPR-Cas13a and RT-loop-mediated isothermal amplification (RT-LAMP). In collaboration with an NHS diagnostic testing lab, we report the performance of the overall workflow and detection of SARS-CoV-2 in patient samples using RT-qPCR, CRISPR-Cas13a, and RT-LAMP. The validated RNA extraction and RT-qPCR platform has been installed in NHS diagnostic labs, increasing testing capacity by 1000 samples per day. Twit Text FOAVip A role for Biofoundries in rapid development and validation of automated SARS-CoV-2 clinical diagnostics ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=32900994 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32900994 #FOAvip English Wikipedia <ref>{{Cite pmid|32900994}}</ref> A role for Biofoundries in rapid development and validation of automated SARS-CoV-2 clinical diagnostics #MMPMID32900994 Crone MA; Priestman M; Ciechonska M; Jensen K; Sharp DJ; Anand A; Randell P; Storch M; Freemont PS Nat Commun 2020[Sep]; 11 (1): 4464 PMID32900994show ga The SARS-CoV-2 pandemic has shown how a rapid rise in demand for patient and community sample testing can quickly overwhelm testing capability globally. With most diagnostic infrastructure dependent on specialized instruments, their exclusive reagent supplies quickly become bottlenecks, creating an urgent need for approaches to boost testing capacity. We address this challenge by refocusing the London Biofoundry onto the development of alternative testing pipelines. Here, we present a reagent-agnostic automated SARS-CoV-2 testing platform that can be quickly deployed and scaled. Using an in-house-generated, open-source, MS2-virus-like particle (VLP) SARS-CoV-2 standard, we validate RNA extraction and RT-qPCR workflows as well as two detection assays based on CRISPR-Cas13a and RT-loop-mediated isothermal amplification (RT-LAMP). In collaboration with an NHS diagnostic testing lab, we report the performance of the overall workflow and detection of SARS-CoV-2 in patient samples using RT-qPCR, CRISPR-Cas13a, and RT-LAMP. The validated RNA extraction and RT-qPCR platform has been installed in NHS diagnostic labs, increasing testing capacity by 1000 samples per day. |Betacoronavirus/*genetics/isolation & purification[MESH] |Biological Assay[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |CRISPR-Cas Systems[MESH] |Clinical Laboratory Techniques/instrumentation/*methods/standards[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/methods[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/*virology[MESH] |RNA, Viral/analysis/genetics/isolation & purification[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH]A role for Biofoundries in rapid development and validation of automat(epmc).pdf DeepDyve Pubget Overpricing