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10.1073/pnas.2011221117 http://scihub22266oqcxt.onion/10.1073/pnas.2011221117 32900935!7533677!32900935     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2020 ; 117 (39): 24450-24458 Nephropedia Template TP {{tp|p=32900935|t=2020. SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification |pdf=|usr=}}{{32900935}} gab.com Text SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=32900935 #FOAvip The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has had an enormous impact on society worldwide, threatening the lives and livelihoods of many. The effects will continue to grow and worsen if economies begin to open without the proper precautions, including expanded diagnostic capabilities. To address this need for increased testing, we have developed a sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay compatible with current reagents, which utilizes a colorimetric readout in as little as 30 min. A rapid inactivation protocol capable of inactivating virions, as well as endogenous nucleases, was optimized to increase sensitivity and sample stability. This protocol, combined with the RT-LAMP assay, has a sensitivity of at least 50 viral RNA copies per microliter in a sample. To further increase the sensitivity, a purification protocol compatible with this inactivation method was developed. The inactivation and purification protocol, combined with the RT-LAMP assay, brings the sensitivity to at least 1 viral RNA copy per microliter in a sample. This simple inactivation and purification pipeline is inexpensive and compatible with other downstream RNA detection platforms and uses readily available reagents. It should increase the availability of SARS-CoV-2 testing as well as expand the settings in which this testing can be performed. Twit Text FOAVip SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=32900935 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32900935 #FOAvip English Wikipedia <ref>{{Cite pmid|32900935}}</ref> SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification #MMPMID32900935 Rabe BA; Cepko C Proc Natl Acad Sci U S A 2020[Sep]; 117 (39): 24450-24458 PMID32900935show ga The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has had an enormous impact on society worldwide, threatening the lives and livelihoods of many. The effects will continue to grow and worsen if economies begin to open without the proper precautions, including expanded diagnostic capabilities. To address this need for increased testing, we have developed a sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay compatible with current reagents, which utilizes a colorimetric readout in as little as 30 min. A rapid inactivation protocol capable of inactivating virions, as well as endogenous nucleases, was optimized to increase sensitivity and sample stability. This protocol, combined with the RT-LAMP assay, has a sensitivity of at least 50 viral RNA copies per microliter in a sample. To further increase the sensitivity, a purification protocol compatible with this inactivation method was developed. The inactivation and purification protocol, combined with the RT-LAMP assay, brings the sensitivity to at least 1 viral RNA copy per microliter in a sample. This simple inactivation and purification pipeline is inexpensive and compatible with other downstream RNA detection platforms and uses readily available reagents. It should increase the availability of SARS-CoV-2 testing as well as expand the settings in which this testing can be performed. |Betacoronavirus/genetics/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/economics[MESH] |Colorimetry[MESH] |Coronavirus Infections/*diagnosis/economics/virology[MESH] |Genome, Viral/genetics[MESH] |Humans[MESH] |Hydrogen-Ion Concentration[MESH] |Molecular Diagnostic Techniques/economics/*methods[MESH] |Nucleic Acid Amplification Techniques/economics/*methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |Polyproteins[MESH] |RNA Stability[MESH] |RNA, Viral/chemistry/genetics/isolation & purification[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Time Factors[MESH] |Viral Proteins/genetics[MESH]SARS-CoV-2 detection using isothermal amplification and a rapid, inexp(epmc).pdf DeepDyve Pubget Overpricing