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10.1016/j.jcv.2020.104587 http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104587 32898817!7418644!32898817     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2020 ; 132 (ä): 104587 Nephropedia Template TP {{tp|p=32898817|t=2020. Use of a simplified sample processing step without RNA extraction for direct SARS-CoV-2 RT-PCR detection |pdf=|usr=}}{{32898817}} gab.com Text Use of a simplified sample processing step without RNA extraction for direct SARS-CoV-2 RT-PCR detection JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32898817 #FOAvip The severe acute respiratory syndrome coronavirus (SARS-CoV-2) pandemic has resulted in significant shortages of RT-PCR testing supplies including RNA extraction kits. The goal of our study was to determine if a simplified heat-RNA release method would provide comparable detection of SARS-CoV-2 without the need for nucleic acid extraction. RT-PCR results using the ChromaCode HDPCR SARS-CoV-2 were compared using the heat-RNA release method and an automated RNA extraction system (EMAG). The heat-RNA release method correctly identified 94 % (81/86 nasopharyngeal samples) that were positive for SARS-CoV-2. Five samples that were missed by heat-RNA release method had a mean Ct value: 35 using the automated extraction instrument, indicating a very low viral load. Our findings show that a simple heat-RNA release method is a reasonable alternative for the majority of COVID-19 positive patients and can help overcome the cost and availability issues of RNA extraction reagents. Twit Text FOAVip Use of a simplified sample processing step without RNA extraction for direct SARS-CoV-2 RT-PCR detection ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32898817 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32898817 #FOAvip English Wikipedia <ref>{{Cite pmid|32898817}}</ref> Use of a simplified sample processing step without RNA extraction for direct SARS-CoV-2 RT-PCR detection #MMPMID32898817 Barza R; Patel P; Sabatini L; Singh K J Clin Virol 2020[Nov]; 132 (ä): 104587 PMID32898817show ga The severe acute respiratory syndrome coronavirus (SARS-CoV-2) pandemic has resulted in significant shortages of RT-PCR testing supplies including RNA extraction kits. The goal of our study was to determine if a simplified heat-RNA release method would provide comparable detection of SARS-CoV-2 without the need for nucleic acid extraction. RT-PCR results using the ChromaCode HDPCR SARS-CoV-2 were compared using the heat-RNA release method and an automated RNA extraction system (EMAG). The heat-RNA release method correctly identified 94 % (81/86 nasopharyngeal samples) that were positive for SARS-CoV-2. Five samples that were missed by heat-RNA release method had a mean Ct value: 35 using the automated extraction instrument, indicating a very low viral load. Our findings show that a simple heat-RNA release method is a reasonable alternative for the majority of COVID-19 positive patients and can help overcome the cost and availability issues of RNA extraction reagents. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |RNA, Viral/analysis/*genetics[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH]Use of a simplified sample processing step without RNA extraction for (epmc).pdf DeepDyve Pubget Overpricing