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10.1080/22221751.2020.1815589 http://scihub22266oqcxt.onion/10.1080/22221751.2020.1815589 32893735!7534347!32893735     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Emerg+Microbes+Infect 2020 ; 9 (1): 2105-2113 Nephropedia Template TP {{tp|p=32893735|t=2020. Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells |pdf=|usr=}}{{32893735}} gab.com Text Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32893735 #FOAvip The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based on VSV-SARS-CoV-2-Sdel18 pseudovirus and hACE2-overexpressing BHK21 cells (BHK21-hACE2 cells). The experimental results can be obtained by automatically counting the number of EGFP-positive cells at 12 h after infection, making the assay convenient and high-throughput. The serum neutralizing titer measured by the VSV-SARS-CoV-2-Sdel18 pseudovirus assay has a good correlation with that measured by the wild type SARS-CoV-2 assay. Seven neutralizing monoclonal antibodies targeting the receptor binding domain (RBD) of the SARS-CoV-2 S protein were obtained. This efficient and reliable pseudovirus assay model could facilitate the development of new drugs and vaccines. Twit Text FOAVip Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32893735 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32893735 #FOAvip English Wikipedia <ref>{{Cite pmid|32893735}}</ref> Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells #MMPMID32893735 Xiong HL; Wu YT; Cao JL; Yang R; Liu YX; Ma J; Qiao XY; Yao XY; Zhang BH; Zhang YL; Hou WH; Shi Y; Xu JJ; Zhang L; Wang SJ; Fu BR; Yang T; Ge SX; Zhang J; Yuan Q; Huang BY; Li ZY; Zhang TY; Xia NS Emerg Microbes Infect 2020[Dec]; 9 (1): 2105-2113 PMID32893735show ga The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human society. A convenient and reliable neutralization assay is very important for the development of vaccines and novel drugs. In this study, a G protein-deficient vesicular stomatitis virus (VSVdG) bearing a truncated spike protein (S with C-terminal 18 amino acid truncation) was compared to that bearing the full-length spike protein of SARS-CoV-2 and showed much higher efficiency. A neutralization assay was established based on VSV-SARS-CoV-2-Sdel18 pseudovirus and hACE2-overexpressing BHK21 cells (BHK21-hACE2 cells). The experimental results can be obtained by automatically counting the number of EGFP-positive cells at 12 h after infection, making the assay convenient and high-throughput. The serum neutralizing titer measured by the VSV-SARS-CoV-2-Sdel18 pseudovirus assay has a good correlation with that measured by the wild type SARS-CoV-2 assay. Seven neutralizing monoclonal antibodies targeting the receptor binding domain (RBD) of the SARS-CoV-2 S protein were obtained. This efficient and reliable pseudovirus assay model could facilitate the development of new drugs and vaccines. |Animals[MESH] |Antibodies, Monoclonal/immunology[MESH] |Antibodies, Neutralizing/*blood/immunology[MESH] |Antibodies, Viral/*blood/immunology[MESH] |Betacoronavirus/*immunology[MESH] |COVID-19[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*diagnosis[MESH] |Cricetinae[MESH] |Neutralization Tests/*methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |SARS-CoV-2[MESH] |Spike Glycoprotein, Coronavirus/genetics/*immunology[MESH] |Vero Cells[MESH]Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesi(epmc).pdf DeepDyve Pubget Overpricing