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10.1007/s00705-020-04796-z

http://scihub22266oqcxt.onion/10.1007/s00705-020-04796-z
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suck abstract from ncbi


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pmid32892248      Arch+Virol 2020 ; 165 (12): 2883-2889
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  • Prevalence and phylogenetic analysis of porcine deltacoronavirus in Sichuan province, China #MMPMID32892248
  • Feng Y; Xu Z; Zhu L
  • Arch Virol 2020[Dec]; 165 (12): 2883-2889 PMID32892248show ga
  • In order to understand the prevalence and genetic diversity of porcine deltacoronavirus (PDCoV) in diarrhoeal pigs in Sichuan province, 634 clinical samples were collected from individual pigs with diarrhoea in 13 regions of Sichuan province, China, from January 2017 and June 2019. The detection results showed that the infection rate of PDCoV was relatively low in diarrhoeal pigs, 13.25% (84/634), but the infection rate of PEDV (porcine epidemic diarrhea virus) was high, 32.18% (204/634). Coinfection with PEDV was common (55.95%, 47/84) in PDCoV-infected diarrhoeal pigs. Additionally, the chance of PDCoV infection was 2.77 times higher in suckling piglets than in sows, and about 3.30 times higher in spring and winter than in summer. PDCoV/PEDV coinfection was 75% less likely in sows than in suckling piglets. The complete genomes of four Sichuan PDCoV strains were sequenced and analysed. There were some insertion-deletion signatures in the whole genome sequences of four strains, including a 6-nt deletion in the non-structural gene 2 region, a 9-nt insertion in the non-structural gene 3 region, a 3-nt deletion in the S gene region, and a distinguishing 11-nt deletion in the 3'UTR region. Phylogenetic analysis based on complete genome sequences revealed that the PDCoV Sichuan strains were closely related to other Chinese PDCoV reference strains; however, phylogenetic analysis based on S gene sequences showed that the CH/SC/2019 strain clustered in a large clade with strains from the USA, Japan, and Korea. These data advance our understanding of the genetic diversity and evolutionary characteristics of PDCoV in China and may contribute to vaccine development.
  • |*Genome, Viral[MESH]
  • |Animals[MESH]
  • |China[MESH]
  • |Coinfection/veterinary[MESH]
  • |Coronavirus Infections/epidemiology/*veterinary/virology[MESH]
  • |Diarrhea/epidemiology/*veterinary/virology[MESH]
  • |Feces/virology[MESH]
  • |Phylogeny[MESH]
  • |Porcine epidemic diarrhea virus/genetics/*physiology[MESH]
  • |Prevalence[MESH]
  • |Sus scrofa/virology[MESH]
  • |Swine[MESH]
  • |Swine Diseases/*epidemiology/virology[MESH]


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