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10.1016/j.jviromet.2020.113965 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.113965 32891677!7468345!32891677     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2020 ; 286 (ä): 113965 Nephropedia Template TP {{tp|p=32891677|t=2020. Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA |pdf=|usr=}}{{32891677}} gab.com Text Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32891677 #FOAvip Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capacities delivering results in a rapid and reliable manner are a prerequisite. Here, we provide and validate such a rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA, termed COVID-quick-DET. This straightforward method operates with simple proteinase K treatment and repetitive heating steps with a sensitivity of 94.6% in head-to-head comparisons with kit-based isolation methods. This result is supported by data obtained from serially diluted SARS-CoV-2 virus stocks. Given its cost- and time-effective operation, COVID-quick-DET might be best suited for countries with general shortage or temporary acute scarcity of resources and equipment. Twit Text FOAVip Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32891677 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32891677 #FOAvip English Wikipedia <ref>{{Cite pmid|32891677}}</ref> Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA #MMPMID32891677 Michel D; Danzer KM; Gross R; Conzelmann C; Muller JA; Freischmidt A; Weishaupt JH; Heller S; Munch J; Michel M; Stamminger T; Kleger A; Otto M J Virol Methods 2020[Dec]; 286 (ä): 113965 PMID32891677show ga Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capacities delivering results in a rapid and reliable manner are a prerequisite. Here, we provide and validate such a rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA, termed COVID-quick-DET. This straightforward method operates with simple proteinase K treatment and repetitive heating steps with a sensitivity of 94.6% in head-to-head comparisons with kit-based isolation methods. This result is supported by data obtained from serially diluted SARS-CoV-2 virus stocks. Given its cost- and time-effective operation, COVID-quick-DET might be best suited for countries with general shortage or temporary acute scarcity of resources and equipment. |*Reagent Kits, Diagnostic[MESH] |Betacoronavirus/*genetics/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |Diagnostic Tests, Routine[MESH] |Endopeptidase K/chemistry[MESH] |Heating[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/*virology[MESH] |RNA, Viral/genetics/*isolation & purification[MESH] |SARS-CoV-2[MESH]Rapid, convenient and efficient kit-independent detection of SARS-CoV-(epmc).pdf DeepDyve Pubget Overpricing