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10.1016/j.phymed.2020.153296 http://scihub22266oqcxt.onion/10.1016/j.phymed.2020.153296 32890913!7395229!32890913     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32890913&cmd=llinks): Failed to open stream: HTTP request failed! 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Phillyrin (KD-1) exerts anti-viral and anti-inflammatory activities against novel coronavirus (SARS-CoV-2) and human coronavirus 229E (HCoV-229E) by suppressing the nuclear factor kappa B (NF-kappaB) signaling pathway |pdf=|usr=}}{{32890913}} gab.com Text Phillyrin (KD-1) exerts anti-viral and anti-inflammatory activities against novel coronavirus (SARS-CoV-2) and human coronavirus 229E (HCoV-229E) by suppressing the nuclear factor kappa B (NF-kappaB) signaling pathway JO: Phytomedicine http://www.kidney.de/pget.php?&tw=1&pmid=32890913 #FOAvip BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has extensively and rapidly spread in the world, causing an outbreak of acute infectious pneumonia. However, no specific antiviral drugs or vaccines can be used. Phillyrin (KD-1), a representative ingredient of Forsythia suspensa, possesses anti-inflammatory, anti-oxidant, and antiviral activities. However, little is known about the antiviral abilities and mechanism of KD-1 against SARS-CoV-2 and human coronavirus 229E (HCoV-229E). PURPOSE: The study was designed to investigate the antiviral and anti-inflammatory activities of KD-1 against the novel SARS-CoV-2 and HCoV-229E and its potential effect in regulating host immune response in vitro. METHODS: The antiviral activities of KD-1 against SARS-CoV-2 and HCoV-229E were assessed in Vero E6 cells using cytopathic effect and plaque-reduction assay. Proinflammatory cytokine expression levels upon infection with SARS-CoV-2 and HCoV-229E infection in Huh-7 cells were measured by real-time quantitative PCR assays. Western blot assay was used to determine the protein expression of nuclear factor kappa B (NF-kappaB) p65, p-NF-kappaB p65, IkappaBalpha, and p-IkappaBalpha in Huh-7 cells, which are the key targets of the NF-kappaB pathway. RESULTS: KD-1 could significantly inhibit SARS-CoV-2 and HCoV-229E replication in vitro. KD-1 could also markedly reduce the production of proinflammatory cytokines (TNF-alpha, IL-6, IL-1beta, MCP-1, and IP-10) at the mRNA levels. Moreover, KD-1 could significantly reduce the protein expression of p-NF-kappaB p65, NF-kappaB p65, and p-IkappaBalpha, while increasing the expression of IkappaBalpha in Huh-7 cells. CONCLUSIONS: KD-1 could significantly inhibit virus proliferation in vitro, the up-regulated expression of proinflammatory cytokines induced by SARS-CoV-2 and HCoV-229E by regulating the activity of the NF-small ka, CyrillicB signaling pathway. Our findings indicated that KD-1 protected against virus attack and can thus be used as a novel strategy for controlling the coronavirus disease 2019. Twit Text FOAVip Phillyrin (KD-1) exerts anti-viral and anti-inflammatory activities against novel coronavirus (SARS-CoV-2) and human coronavirus 229E (HCoV-229E) by suppressing the nuclear factor kappa B (NF-kappaB) signaling pathway ????Phytomedicine http://www.kidney.de/pget.php?&tw=1&pmid=32890913 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32890913 #FOAvip English Wikipedia <ref>{{Cite pmid|32890913}}</ref> Phillyrin (KD-1) exerts anti-viral and anti-inflammatory activities against novel coronavirus (SARS-CoV-2) and human coronavirus 229E (HCoV-229E) by suppressing the nuclear factor kappa B (NF-kappaB) signaling pathway #MMPMID32890913 Ma Q; Li R; Pan W; Huang W; Liu B; Xie Y; Wang Z; Li C; Jiang H; Huang J; Shi Y; Dai J; Zheng K; Li X; Hui M; Fu L; Yang Z Phytomedicine 2020[Nov]; 78 (ä): 153296 PMID32890913show ga BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has extensively and rapidly spread in the world, causing an outbreak of acute infectious pneumonia. However, no specific antiviral drugs or vaccines can be used. Phillyrin (KD-1), a representative ingredient of Forsythia suspensa, possesses anti-inflammatory, anti-oxidant, and antiviral activities. However, little is known about the antiviral abilities and mechanism of KD-1 against SARS-CoV-2 and human coronavirus 229E (HCoV-229E). PURPOSE: The study was designed to investigate the antiviral and anti-inflammatory activities of KD-1 against the novel SARS-CoV-2 and HCoV-229E and its potential effect in regulating host immune response in vitro. METHODS: The antiviral activities of KD-1 against SARS-CoV-2 and HCoV-229E were assessed in Vero E6 cells using cytopathic effect and plaque-reduction assay. Proinflammatory cytokine expression levels upon infection with SARS-CoV-2 and HCoV-229E infection in Huh-7 cells were measured by real-time quantitative PCR assays. Western blot assay was used to determine the protein expression of nuclear factor kappa B (NF-kappaB) p65, p-NF-kappaB p65, IkappaBalpha, and p-IkappaBalpha in Huh-7 cells, which are the key targets of the NF-kappaB pathway. RESULTS: KD-1 could significantly inhibit SARS-CoV-2 and HCoV-229E replication in vitro. KD-1 could also markedly reduce the production of proinflammatory cytokines (TNF-alpha, IL-6, IL-1beta, MCP-1, and IP-10) at the mRNA levels. Moreover, KD-1 could significantly reduce the protein expression of p-NF-kappaB p65, NF-kappaB p65, and p-IkappaBalpha, while increasing the expression of IkappaBalpha in Huh-7 cells. CONCLUSIONS: KD-1 could significantly inhibit virus proliferation in vitro, the up-regulated expression of proinflammatory cytokines induced by SARS-CoV-2 and HCoV-229E by regulating the activity of the NF-small ka, CyrillicB signaling pathway. Our findings indicated that KD-1 protected against virus attack and can thus be used as a novel strategy for controlling the coronavirus disease 2019. |*Coronavirus Infections/metabolism/virology[MESH] |*Pandemics[MESH] |*Pneumonia, Viral/metabolism/virology[MESH] |Animals[MESH] |Anti-Inflammatory Agents/*pharmacology[MESH] |Antiviral Agents/*pharmacology[MESH] |Betacoronavirus/*drug effects[MESH] |COVID-19[MESH] |Chlorocebus aethiops[MESH] |Coronavirus 229E, Human/*drug effects[MESH] |Coronavirus/drug effects[MESH] |Cytokines/metabolism[MESH] |Forsythia/chemistry[MESH] |Glucosides/*pharmacology[MESH] |Humans[MESH] |NF-kappa B/*metabolism[MESH] |Phytotherapy[MESH] |Plant Extracts/pharmacology[MESH] |SARS-CoV-2[MESH] |Severe Acute Respiratory Syndrome/virology[MESH] |Signal Transduction/drug effects[MESH] |Vero Cells[MESH]Phillyrin (KD-1) exerts anti-viral and anti-inflammatory activities ag(epmc).pdf DeepDyve Pubget Overpricing