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10.1016/j.bjid.2020.08.001 http://scihub22266oqcxt.onion/10.1016/j.bjid.2020.08.001 32888905!7458056!32888905     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Braz+J+Infect+Dis 2020 ; 24 (5): 422-427 Nephropedia Template TP {{tp|p=32888905|t=2020. Saliva is a reliable, non-invasive specimen for SARS-CoV-2 detection |pdf=|usr=}}{{32888905}} gab.com Text Saliva is a reliable, non-invasive specimen for SARS-CoV-2 detection JO: Braz J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32888905 #FOAvip BACKGROUND: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19). Although Real Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) of respiratory specimens is the gold standard test for detection of SARS-CoV-2 infection, collecting nasopharyngeal swabs causes discomfort to patients and may represent considerable risk for healthcare workers. The use of saliva as a diagnostic sample has several advantages. OBJECTIVES: The aim of this study was to validate the use of saliva as a biological sample for diagnosis of COVID-19. METHODS: This study was conducted at Infectious Diseases Research Laboratory (LAPI), in Salvador, Brazil. Participants presenting with signs/symptoms suggesting SARS-CoV-2 infection underwent a nasopharyngeal swab (NPS) and/or oropharyngeal swab (OPS), and saliva collection. Saliva samples were diluted in PBS, followed by RNA isolation and RT-Real Time PCR for SARS-CoV-2. Results of conventional vs saliva samples testing were compared. Statistical analyses were performed using Statistical Package for the Social Sciences software (SPSS) version 18.0. RESULTS: One hundred fifty-five participants were recruited and samples pairs of NPS/OPS and saliva were collected. The sensitivity and specificity of RT-PCR using saliva samples were 94.4% (95% CI 86.4-97.8) and 97.62% (95% CI 91.7-99.3), respectively. There was an overall high agreement (96.1%) between the two tests. CONCLUSIONS: Use of self-collected saliva samples is an easy, convenient, and low-cost alternative to conventional NP swab-based molecular tests. These results may allow a broader use of molecular tests for management of COVID19 pandemic, especially in resources-limited settings. Twit Text FOAVip Saliva is a reliable, non-invasive specimen for SARS-CoV-2 detection ????Braz J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32888905 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32888905 #FOAvip English Wikipedia <ref>{{Cite pmid|32888905}}</ref> Saliva is a reliable, non-invasive specimen for SARS-CoV-2 detection #MMPMID32888905 Vaz SN; Santana DS; Netto EM; Pedroso C; Wang WK; Santos FDA; Brites C Braz J Infect Dis 2020[Sep]; 24 (5): 422-427 PMID32888905show ga BACKGROUND: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19). Although Real Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) of respiratory specimens is the gold standard test for detection of SARS-CoV-2 infection, collecting nasopharyngeal swabs causes discomfort to patients and may represent considerable risk for healthcare workers. The use of saliva as a diagnostic sample has several advantages. OBJECTIVES: The aim of this study was to validate the use of saliva as a biological sample for diagnosis of COVID-19. METHODS: This study was conducted at Infectious Diseases Research Laboratory (LAPI), in Salvador, Brazil. Participants presenting with signs/symptoms suggesting SARS-CoV-2 infection underwent a nasopharyngeal swab (NPS) and/or oropharyngeal swab (OPS), and saliva collection. Saliva samples were diluted in PBS, followed by RNA isolation and RT-Real Time PCR for SARS-CoV-2. Results of conventional vs saliva samples testing were compared. Statistical analyses were performed using Statistical Package for the Social Sciences software (SPSS) version 18.0. RESULTS: One hundred fifty-five participants were recruited and samples pairs of NPS/OPS and saliva were collected. The sensitivity and specificity of RT-PCR using saliva samples were 94.4% (95% CI 86.4-97.8) and 97.62% (95% CI 91.7-99.3), respectively. There was an overall high agreement (96.1%) between the two tests. CONCLUSIONS: Use of self-collected saliva samples is an easy, convenient, and low-cost alternative to conventional NP swab-based molecular tests. These results may allow a broader use of molecular tests for management of COVID19 pandemic, especially in resources-limited settings. |*Coronavirus Infections/epidemiology[MESH] |*Pandemics[MESH] |*Pneumonia, Viral/epidemiology[MESH] |Betacoronavirus[MESH] |Brazil[MESH] |COVID-19[MESH] |Clinical Laboratory Techniques[MESH] |Humans[MESH] |SARS-CoV-2[MESH]Saliva is a reliable, non-invasive specimen for SARS-CoV-2 detection (epmc).pdf DeepDyve Pubget Overpricing