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10.1074/jbc.RA120.015434 http://scihub22266oqcxt.onion/10.1074/jbc.RA120.015434 32883809!7667971!32883809     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 2020 ; 295 (46): 15438-15453 Nephropedia Template TP {{tp|p=32883809|t=2020. A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits |pdf=|usr=}}{{32883809}} gab.com Text A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=32883809 #FOAvip Widespread testing for the presence of the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in individuals remains vital for controlling the COVID-19 pandemic prior to the advent of an effective treatment. Challenges in testing can be traced to an initial shortage of supplies, expertise, and/or instrumentation necessary to detect the virus by quantitative RT-PCR (RT-qPCR), the most robust, sensitive, and specific assay currently available. Here we show that academic biochemistry and molecular biology laboratories equipped with appropriate expertise and infrastructure can replicate commercially available SARS-CoV-2 RT-qPCR test kits and backfill pipeline shortages. The Georgia Tech COVID-19 Test Kit Support Group, composed of faculty, staff, and trainees across the biotechnology quad at Georgia Institute of Technology, synthesized multiplexed primers and probes and formulated a master mix composed of enzymes and proteins produced in-house. Our in-house kit compares favorably with a commercial product used for diagnostic testing. We also developed an environmental testing protocol to readily monitor surfaces for the presence of SARS-CoV-2. Our blueprint should be readily reproducible by research teams at other institutions, and our protocols may be modified and adapted to enable SARS-CoV-2 detection in more resource-limited settings. Twit Text FOAVip A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=32883809 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32883809 #FOAvip English Wikipedia <ref>{{Cite pmid|32883809}}</ref> A blueprint for academic laboratories to produce SARS-CoV-2 quantitative RT-PCR test kits #MMPMID32883809 Mascuch SJ; Fakhretaha-Aval S; Bowman JC; Ma MTH; Thomas G; Bommarius B; Ito C; Zhao L; Newnam GP; Matange KR; Thapa HR; Barlow B; Donegan RK; Nguyen NA; Saccuzzo EG; Obianyor CT; Karunakaran SC; Pollet P; Rothschild-Mancinelli B; Mestre-Fos S; Guth-Metzler R; Bryksin AV; Petrov AS; Hazell M; Ibberson CB; Penev PI; Mannino RG; Lam WA; Garcia AJ; Kubanek J; Agarwal V; Hud NV; Glass JB; Williams LD; Lieberman RL J Biol Chem 2020[Nov]; 295 (46): 15438-15453 PMID32883809show ga Widespread testing for the presence of the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in individuals remains vital for controlling the COVID-19 pandemic prior to the advent of an effective treatment. Challenges in testing can be traced to an initial shortage of supplies, expertise, and/or instrumentation necessary to detect the virus by quantitative RT-PCR (RT-qPCR), the most robust, sensitive, and specific assay currently available. Here we show that academic biochemistry and molecular biology laboratories equipped with appropriate expertise and infrastructure can replicate commercially available SARS-CoV-2 RT-qPCR test kits and backfill pipeline shortages. The Georgia Tech COVID-19 Test Kit Support Group, composed of faculty, staff, and trainees across the biotechnology quad at Georgia Institute of Technology, synthesized multiplexed primers and probes and formulated a master mix composed of enzymes and proteins produced in-house. Our in-house kit compares favorably with a commercial product used for diagnostic testing. We also developed an environmental testing protocol to readily monitor surfaces for the presence of SARS-CoV-2. Our blueprint should be readily reproducible by research teams at other institutions, and our protocols may be modified and adapted to enable SARS-CoV-2 detection in more resource-limited settings. |*Technology Transfer[MESH] |Biotechnology/methods[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |Reagent Kits, Diagnostic/*economics/supply & distribution[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH]A blueprint for academic laboratories to produce SARS-CoV-2 quantitati(epmc).pdf DeepDyve Pubget Overpricing