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10.1038/s41467-020-18149-6

http://scihub22266oqcxt.onion/10.1038/s41467-020-18149-6
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32879306!7467924!32879306
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suck abstract from ncbi


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pmid32879306      Nat+Commun 2020 ; 11 (1): 4400
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  • Ocular conjunctival inoculation of SARS-CoV-2 can cause mild COVID-19 in rhesus macaques #MMPMID32879306
  • Deng W; Bao L; Gao H; Xiang Z; Qu Y; Song Z; Gong S; Liu J; Liu J; Yu P; Qi F; Xu Y; Li F; Xiao C; Lv Q; Xue J; Wei Q; Liu M; Wang G; Wang S; Yu H; Chen T; Liu X; Zhao W; Han Y; Qin C
  • Nat Commun 2020[Sep]; 11 (1): 4400 PMID32879306show ga
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly transmitted through the respiratory route, but potential extra-respiratory routes of SARS-CoV-2 transmission remain uncertain. Here we inoculated five rhesus macaques with 1 x 10(6) TCID(50) of SARS-CoV-2 conjunctivally (CJ), intratracheally (IT), and intragastrically (IG). Nasal and throat swabs collected from CJ and IT had detectable viral RNA at 1-7 days post-inoculation (dpi). Viral RNA was detected in anal swabs from only the IT group at 1-7 dpi. Viral RNA was undetectable in tested swabs and tissues after intragastric inoculation. The CJ infected animal had a higher viral load in the nasolacrimal system than the IT infected animal but also showed mild interstitial pneumonia, suggesting distinct virus distributions. This study shows that infection via the conjunctival route is possible in non-human primates; further studies are necessary to compare the relative risk and pathogenesis of infection through these different routes in more detail.
  • |*Disease Models, Animal[MESH]
  • |Animals[MESH]
  • |Antibodies, Viral[MESH]
  • |Betacoronavirus/genetics/isolation & purification/*physiology[MESH]
  • |COVID-19[MESH]
  • |Conjunctiva/*virology[MESH]
  • |Coronavirus Infections/pathology/*virology[MESH]
  • |Intestine, Large/virology[MESH]
  • |Lung/pathology/virology[MESH]
  • |Macaca mulatta[MESH]
  • |Male[MESH]
  • |Nasal Cavity/virology[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/pathology/*virology[MESH]
  • |RNA, Viral/analysis/genetics[MESH]
  • |SARS-CoV-2[MESH]
  • |Trachea/virology[MESH]
  • |Viral Load[MESH]


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