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10.3390/v12090960 http://scihub22266oqcxt.onion/10.3390/v12090960 32872469!7552073!32872469     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2020 ; 12 (9): ä Nephropedia Template TP {{tp|p=32872469|t=2020. The Virome of Acute Respiratory Diseases in Individuals at Risk of Zoonotic Infections |pdf=|usr=}}{{32872469}} gab.com Text The Virome of Acute Respiratory Diseases in Individuals at Risk of Zoonotic Infections JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32872469 #FOAvip The ongoing coronavirus disease 2019 (COVID-19) pandemic emphasizes the need to actively study the virome of unexplained respiratory diseases. We performed viral metagenomic next-generation sequencing (mNGS) analysis of 91 nasal-throat swabs from individuals working with animals and with acute respiratory diseases. Fifteen virus RT-PCR-positive samples were included as controls, while the other 76 samples were RT-PCR negative for a wide panel of respiratory pathogens. Eukaryotic viruses detected by mNGS were then screened by PCR (using primers based on mNGS-derived contigs) in all samples to compare viral detection by mNGS versus PCR and assess the utility of mNGS in routine diagnostics. mNGS identified expected human rhinoviruses, enteroviruses, influenza A virus, coronavirus OC43, and respiratory syncytial virus (RSV) A in 13 of 15 (86.7%) positive control samples. Additionally, rotavirus, torque teno virus, human papillomavirus, human betaherpesvirus 7, cyclovirus, vientovirus, gemycircularvirus, and statovirus were identified through mNGS. Notably, complete genomes of novel cyclovirus, gemycircularvirus, and statovirus were genetically characterized. Using PCR screening, the novel cyclovirus was additionally detected in 5 and the novel gemycircularvirus in 12 of the remaining samples included for mNGS analysis. Our studies therefore provide pioneering data of the virome of acute-respiratory diseases from individuals at risk of zoonotic infections. The mNGS protocol/pipeline applied here is sensitive for the detection of a variety of viruses, including novel ones. More frequent detections of the novel viruses by PCR than by mNGS on the same samples suggests that PCR remains the most sensitive diagnostic test for viruses whose genomes are known. The detection of novel viruses expands our understanding of the respiratory virome of animal-exposed humans and warrant further studies. Twit Text FOAVip The Virome of Acute Respiratory Diseases in Individuals at Risk of Zoonotic Infections ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32872469 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32872469 #FOAvip English Wikipedia <ref>{{Cite pmid|32872469}}</ref> The Virome of Acute Respiratory Diseases in Individuals at Risk of Zoonotic Infections #MMPMID32872469 Thi Kha Tu N; Thi Thu Hong N; Thi Han Ny N; My Phuc T; Thi Thanh Tam P; Doorn HRV; Dang Trung Nghia H; Thao Huong D; An Han D; Thi Thu Ha L; Deng X; Thwaites G; Delwart E; Virtala AK; Vapalahti O; Baker S; Van Tan L Viruses 2020[Aug]; 12 (9): ä PMID32872469show ga The ongoing coronavirus disease 2019 (COVID-19) pandemic emphasizes the need to actively study the virome of unexplained respiratory diseases. We performed viral metagenomic next-generation sequencing (mNGS) analysis of 91 nasal-throat swabs from individuals working with animals and with acute respiratory diseases. Fifteen virus RT-PCR-positive samples were included as controls, while the other 76 samples were RT-PCR negative for a wide panel of respiratory pathogens. Eukaryotic viruses detected by mNGS were then screened by PCR (using primers based on mNGS-derived contigs) in all samples to compare viral detection by mNGS versus PCR and assess the utility of mNGS in routine diagnostics. mNGS identified expected human rhinoviruses, enteroviruses, influenza A virus, coronavirus OC43, and respiratory syncytial virus (RSV) A in 13 of 15 (86.7%) positive control samples. Additionally, rotavirus, torque teno virus, human papillomavirus, human betaherpesvirus 7, cyclovirus, vientovirus, gemycircularvirus, and statovirus were identified through mNGS. Notably, complete genomes of novel cyclovirus, gemycircularvirus, and statovirus were genetically characterized. Using PCR screening, the novel cyclovirus was additionally detected in 5 and the novel gemycircularvirus in 12 of the remaining samples included for mNGS analysis. Our studies therefore provide pioneering data of the virome of acute-respiratory diseases from individuals at risk of zoonotic infections. The mNGS protocol/pipeline applied here is sensitive for the detection of a variety of viruses, including novel ones. More frequent detections of the novel viruses by PCR than by mNGS on the same samples suggests that PCR remains the most sensitive diagnostic test for viruses whose genomes are known. The detection of novel viruses expands our understanding of the respiratory virome of animal-exposed humans and warrant further studies. |Animals[MESH] |COVID-19[MESH] |Coronavirus Infections/diagnosis/virology[MESH] |Coronavirus/genetics/isolation & purification[MESH] |High-Throughput Nucleotide Sequencing/methods[MESH] |Humans[MESH] |Metagenome[MESH] |Metagenomics/methods[MESH] |Pandemics[MESH] |Phylogeny[MESH] |Pneumonia, Viral/diagnosis/virology[MESH] |Respiratory Tract Infections/diagnosis/*virology[MESH] |Virus Diseases/diagnosis/*virology[MESH]The Virome of Acute Respiratory Diseases in Individuals at Risk of Zoo(epmc).pdf DeepDyve Pubget Overpricing