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10.1080/14760584.2020.1811091

http://scihub22266oqcxt.onion/10.1080/14760584.2020.1811091
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suck abstract from ncbi


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pmid32869699      Expert+Rev+Vaccines 2020 ; 19 (9): 871-885
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  • Designing an efficient multi-epitope vaccine displaying interactions with diverse HLA molecules for an efficient humoral and cellular immune response to prevent COVID-19 infection #MMPMID32869699
  • Mahapatra SR; Sahoo S; Dehury B; Raina V; Patro S; Misra N; Suar M
  • Expert Rev Vaccines 2020[Sep]; 19 (9): 871-885 PMID32869699show ga
  • BACKGROUND: The novel SARS-CoV-2 coronavirus, the causative agent of the ongoing pandemic COVID-19 disease continues to infect people globally and has infected millions of humans worldwide. However, no effective vaccine against this virus exists. METHOD: Using Immunoinformatics, epitopic sequences from multiple glycoproteins that play crucial role in pathogenesis were identified. Particularly, epitopes were mapped from conserved receptor-binding domain of spike protein which have been experimentally validated in SARS-CoV-1 as a promising target for vaccine development. RESULTS: A multi-epitopic vaccine construct comprising of B-cell, CTL, HTL epitopes was developed along with fusion of adjuvant and linkers. The epitopes identified herein are reported for the first time and were predicted to be highly antigenic, stable, nonallergen, nontoxic and displayed conservation across several SARS-CoV-2 isolates from different countries. Additionally, the epitopes associated with maximum HLA alleles and population coverage analysis shows the proposed epitopes would be a relevant representative of large proportion of the world population. A reliable three-dimensional structure of the vaccine construct was developed. Consequently, docking and molecular-dynamics simulation ensured the stable interaction between vaccine and innate-immune receptor.
  • |*Immunity, Cellular[MESH]
  • |*Immunity, Humoral[MESH]
  • |Alleles[MESH]
  • |Betacoronavirus/*immunology[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Vaccines[MESH]
  • |Computational Biology[MESH]
  • |Coronavirus Infections/genetics/immunology/*prevention & control/virology[MESH]
  • |Epitope Mapping[MESH]
  • |Epitopes, B-Lymphocyte/immunology[MESH]
  • |Epitopes, T-Lymphocyte/immunology[MESH]
  • |HLA Antigens/genetics/*immunology[MESH]
  • |Humans[MESH]
  • |Immunogenicity, Vaccine[MESH]
  • |Molecular Docking Simulation[MESH]
  • |Molecular Dynamics Simulation[MESH]
  • |Pandemics/*prevention & control[MESH]
  • |Pneumonia, Viral/*prevention & control/virology[MESH]
  • |Protein Binding[MESH]
  • |SARS-CoV-2[MESH]
  • |Spike Glycoprotein, Coronavirus/immunology[MESH]


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