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10.1073/pnas.2014739117 http://scihub22266oqcxt.onion/10.1073/pnas.2014739117 32868442!7502724!32868442     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2020 ; 117 (37): 22727-22735 Nephropedia Template TP {{tp|p=32868442|t=2020. Rapid isothermal amplification and portable detection system for SARS-CoV-2 |pdf=|usr=}}{{32868442}} gab.com Text Rapid isothermal amplification and portable detection system for SARS-CoV-2 JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=32868442 #FOAvip The COVID-19 pandemic provides an urgent example where a gap exists between availability of state-of-the-art diagnostics and current needs. As assay protocols and primer sequences become widely known, many laboratories perform diagnostic tests using methods such as RT-PCR or reverse transcription loop mediated isothermal amplification (RT-LAMP). Here, we report an RT-LAMP isothermal assay for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus and demonstrate the assay on clinical samples using a simple and accessible point-of-care (POC) instrument. We characterized the assay by dipping swabs into synthetic nasal fluid spiked with the virus, moving the swab to viral transport medium (VTM), and sampling a volume of the VTM to perform the RT-LAMP assay without an RNA extraction kit. The assay has a limit of detection (LOD) of 50 RNA copies per muL in the VTM solution within 30 min. We further demonstrate our assay by detecting SARS-CoV-2 viruses from 20 clinical samples. Finally, we demonstrate a portable and real-time POC device to detect SARS-CoV-2 from VTM samples using an additively manufactured three-dimensional cartridge and a smartphone-based reader. The POC system was tested using 10 clinical samples, and was able to detect SARS-CoV-2 from these clinical samples by distinguishing positive samples from negative samples after 30 min. The POC tests are in complete agreement with RT-PCR controls. This work demonstrates an alternative pathway for SARS-CoV-2 diagnostics that does not require conventional laboratory infrastructure, in settings where diagnosis is required at the point of sample collection. Twit Text FOAVip Rapid isothermal amplification and portable detection system for SARS-CoV-2 ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=32868442 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32868442 #FOAvip English Wikipedia <ref>{{Cite pmid|32868442}}</ref> Rapid isothermal amplification and portable detection system for SARS-CoV-2 #MMPMID32868442 Ganguli A; Mostafa A; Berger J; Aydin MY; Sun F; Ramirez SAS; Valera E; Cunningham BT; King WP; Bashir R Proc Natl Acad Sci U S A 2020[Sep]; 117 (37): 22727-22735 PMID32868442show ga The COVID-19 pandemic provides an urgent example where a gap exists between availability of state-of-the-art diagnostics and current needs. As assay protocols and primer sequences become widely known, many laboratories perform diagnostic tests using methods such as RT-PCR or reverse transcription loop mediated isothermal amplification (RT-LAMP). Here, we report an RT-LAMP isothermal assay for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus and demonstrate the assay on clinical samples using a simple and accessible point-of-care (POC) instrument. We characterized the assay by dipping swabs into synthetic nasal fluid spiked with the virus, moving the swab to viral transport medium (VTM), and sampling a volume of the VTM to perform the RT-LAMP assay without an RNA extraction kit. The assay has a limit of detection (LOD) of 50 RNA copies per muL in the VTM solution within 30 min. We further demonstrate our assay by detecting SARS-CoV-2 viruses from 20 clinical samples. Finally, we demonstrate a portable and real-time POC device to detect SARS-CoV-2 from VTM samples using an additively manufactured three-dimensional cartridge and a smartphone-based reader. The POC system was tested using 10 clinical samples, and was able to detect SARS-CoV-2 from these clinical samples by distinguishing positive samples from negative samples after 30 min. The POC tests are in complete agreement with RT-PCR controls. This work demonstrates an alternative pathway for SARS-CoV-2 diagnostics that does not require conventional laboratory infrastructure, in settings where diagnosis is required at the point of sample collection. |Betacoronavirus/genetics/pathogenicity[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/instrumentation/*methods/standards[MESH] |Nasal Mucosa/virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Point-of-Care Testing/*standards[MESH] |Reproducibility of Results[MESH] |Reverse Transcriptase Polymerase Chain Reaction/instrumentation/*methods/standards[MESH] |SARS-CoV-2[MESH]Rapid isothermal amplification and portable detection system for SARS-(epmc).pdf DeepDyve Pubget Overpricing