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10.1177/0145561320953231

http://scihub22266oqcxt.onion/10.1177/0145561320953231
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32865458!7459180!32865458
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suck abstract from ncbi


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pmid32865458      Ear+Nose+Throat+J 2021 ; 100 (2_suppl): 131S-138S
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  • Diagnosis of SARS-CoV-2 by RT-PCR Using Different Sample Sources: Review of the Literature #MMPMID32865458
  • Torretta S; Zuccotti G; Cristofaro V; Ettori J; Solimeno L; Battilocchi L; D'Onghia A; Bonsembiante A; Pignataro L; Marchisio P; Capaccio P
  • Ear Nose Throat J 2021[Apr]; 100 (2_suppl): 131S-138S PMID32865458show ga
  • OBJECTIVE: The most widely used diagnostic technique for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is real-time reverse transcriptase-polymerase chain reaction (RT-PCR). It can be done on different samples: nasopharyngeal swabs (NPS) or oropharyngeal swabs (OPS), and self-collected saliva. However, negative findings do not rule out infection. METHODS: A review was conceived to discuss advantages and limitations of the available diagnostic modalities for nonserologic diagnosis of SARS-CoV-2 based on RT-PCR; the article also proposes some practical suggestions to improve diagnostic reliability. RESULTS: A total of 16 papers (corresponding to 452 patients) of the 56 initially identified were included. Most of the papers describe findings from different samples obtained in limited case series; comparative studies are missing. CONCLUSIONS: Diagnostic accuracy of NPS and OPS is suboptimal and the risk of contaminated aerosol dispersal is not negligible. The SARS-CoV-2 RNA can be found in self-collected saliva specimens of many infected patients within 7 to 10 days after symptom onset. There is an urgent need for comparative trials to define the diagnostic modality of choice. Adequate education and training of health care personnel is mandatory.
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Nasopharynx/*chemistry/virology[MESH]
  • |Oropharynx/*chemistry/virology[MESH]
  • |RNA, Viral/*isolation & purification[MESH]
  • |SARS-CoV-2/*genetics[MESH]
  • |Saliva/*chemistry/virology[MESH]
  • |Sensitivity and Specificity[MESH]


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