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10.1038/s41587-020-0659-0 http://scihub22266oqcxt.onion/10.1038/s41587-020-0659-0 32855547!7740072!32855547     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Biotechnol 2020 ; 38 (10): 1174-1183 Nephropedia Template TP {{tp|p=32855547|t=2020. Evaluation of SARS-CoV-2 serology assays reveals a range of test performance |pdf=|usr=}}{{32855547}} gab.com Text Evaluation of SARS-CoV-2 serology assays reveals a range of test performance JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=32855547 #FOAvip Appropriate use and interpretation of serological tests for assessments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure, infection and potential immunity require accurate data on assay performance. We conducted a head-to-head evaluation of ten point-of-care-style lateral flow assays (LFAs) and two laboratory-based enzyme-linked immunosorbent assays to detect anti-SARS-CoV-2 IgM and IgG antibodies in 5-d time intervals from symptom onset and studied the specificity of each assay in pre-coronavirus disease 2019 specimens. The percent of seropositive individuals increased with time, peaking in the latest time interval tested (>20 d after symptom onset). Test specificity ranged from 84.3% to 100.0% and was predominantly affected by variability in IgM results. LFA specificity could be increased by considering weak bands as negative, but this decreased detection of antibodies (sensitivity) in a subset of SARS-CoV-2 real-time PCR-positive cases. Our results underline the importance of seropositivity threshold determination and reader training for reliable LFA deployment. Although there was no standout serological assay, four tests achieved more than 80% positivity at later time points tested and more than 95% specificity. Twit Text FOAVip Evaluation of SARS-CoV-2 serology assays reveals a range of test performance ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=32855547 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32855547 #FOAvip English Wikipedia <ref>{{Cite pmid|32855547}}</ref> Evaluation of SARS-CoV-2 serology assays reveals a range of test performance #MMPMID32855547 Whitman JD; Hiatt J; Mowery CT; Shy BR; Yu R; Yamamoto TN; Rathore U; Goldgof GM; Whitty C; Woo JM; Gallman AE; Miller TE; Levine AG; Nguyen DN; Bapat SP; Balcerek J; Bylsma SA; Lyons AM; Li S; Wong AW; Gillis-Buck EM; Steinhart ZB; Lee Y; Apathy R; Lipke MJ; Smith JA; Zheng T; Boothby IC; Isaza E; Chan J; Acenas DD 2nd; Lee J; Macrae TA; Kyaw TS; Wu D; Ng DL; Gu W; York VA; Eskandarian HA; Callaway PC; Warrier L; Moreno ME; Levan J; Torres L; Farrington LA; Loudermilk RP; Koshal K; Zorn KC; Garcia-Beltran WF; Yang D; Astudillo MG; Bernstein BE; Gelfand JA; Ryan ET; Charles RC; Iafrate AJ; Lennerz JK; Miller S; Chiu CY; Stramer SL; Wilson MR; Manglik A; Ye CJ; Krogan NJ; Anderson MS; Cyster JG; Ernst JD; Wu AHB; Lynch KL; Bern C; Hsu PD; Marson A Nat Biotechnol 2020[Oct]; 38 (10): 1174-1183 PMID32855547show ga Appropriate use and interpretation of serological tests for assessments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure, infection and potential immunity require accurate data on assay performance. We conducted a head-to-head evaluation of ten point-of-care-style lateral flow assays (LFAs) and two laboratory-based enzyme-linked immunosorbent assays to detect anti-SARS-CoV-2 IgM and IgG antibodies in 5-d time intervals from symptom onset and studied the specificity of each assay in pre-coronavirus disease 2019 specimens. The percent of seropositive individuals increased with time, peaking in the latest time interval tested (>20 d after symptom onset). Test specificity ranged from 84.3% to 100.0% and was predominantly affected by variability in IgM results. LFA specificity could be increased by considering weak bands as negative, but this decreased detection of antibodies (sensitivity) in a subset of SARS-CoV-2 real-time PCR-positive cases. Our results underline the importance of seropositivity threshold determination and reader training for reliable LFA deployment. Although there was no standout serological assay, four tests achieved more than 80% positivity at later time points tested and more than 95% specificity. |*Betacoronavirus/genetics/immunology/isolation & purification[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Antibodies, Viral/blood[MESH] |Biotechnology[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Chromatography, Affinity[MESH] |Clinical Laboratory Techniques/*methods/statistics & numerical data[MESH] |Coronavirus Infections/*diagnosis/epidemiology/immunology[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Female[MESH] |Humans[MESH] |Immunoglobulin G/blood[MESH] |Immunoglobulin M/blood[MESH] |Male[MESH] |Middle Aged[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/epidemiology/immunology[MESH] |Point-of-Care Testing[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH]Evaluation of SARS-CoV-2 serology assays reveals a range of test perfo(epmc).pdf DeepDyve Pubget Overpricing