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10.1186/s12985-020-01402-1 http://scihub22266oqcxt.onion/10.1186/s12985-020-01402-1 32854725!7450977!32854725     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32854725&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virol+J 2020 ; 17 (1): 131 Nephropedia Template TP {{tp|p=32854725|t=2020. Characterization of accessory genes in coronavirus genomes |pdf=|usr=}}{{32854725}} gab.com Text Characterization of accessory genes in coronavirus genomes JO: Virol J http://www.kidney.de/pget.php?&tw=1&pmid=32854725 #FOAvip BACKGROUND: The Covid19 infection is caused by the SARS-CoV-2 virus, a novel member of the coronavirus (CoV) family. CoV genomes code for a ORF1a / ORF1ab polyprotein and four structural proteins widely studied as major drug targets. The genomes also contain a variable number of open reading frames (ORFs) coding for accessory proteins that are not essential for virus replication, but appear to have a role in pathogenesis. The accessory proteins have been less well characterized and are difficult to predict by classical bioinformatics methods. METHODS: We propose a computational tool GOFIX to characterize potential ORFs in virus genomes. In particular, ORF coding potential is estimated by searching for enrichment in motifs of the X circular code, that is known to be over-represented in the reading frames of viral genes. RESULTS: We applied GOFIX to study the SARS-CoV-2 and related genomes including SARS-CoV and SARS-like viruses from bat, civet and pangolin hosts, focusing on the accessory proteins. Our analysis provides evidence supporting the presence of overlapping ORFs 7b, 9b and 9c in all the genomes and thus helps to resolve some differences in current genome annotations. In contrast, we predict that ORF3b is not functional in all genomes. Novel putative ORFs were also predicted, including a truncated form of the ORF10 previously identified in SARS-CoV-2 and a little known ORF overlapping the Spike protein in Civet-CoV and SARS-CoV. CONCLUSIONS: Our findings contribute to characterizing sequence properties of accessory genes of SARS coronaviruses, and especially the newly acquired genes making use of overlapping reading frames. Twit Text FOAVip Characterization of accessory genes in coronavirus genomes ????Virol J http://www.kidney.de/pget.php?&tw=1&pmid=32854725 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32854725 #FOAvip English Wikipedia <ref>{{Cite pmid|32854725}}</ref> Characterization of accessory genes in coronavirus genomes #MMPMID32854725 Michel CJ; Mayer C; Poch O; Thompson JD Virol J 2020[Aug]; 17 (1): 131 PMID32854725show ga BACKGROUND: The Covid19 infection is caused by the SARS-CoV-2 virus, a novel member of the coronavirus (CoV) family. CoV genomes code for a ORF1a / ORF1ab polyprotein and four structural proteins widely studied as major drug targets. The genomes also contain a variable number of open reading frames (ORFs) coding for accessory proteins that are not essential for virus replication, but appear to have a role in pathogenesis. The accessory proteins have been less well characterized and are difficult to predict by classical bioinformatics methods. METHODS: We propose a computational tool GOFIX to characterize potential ORFs in virus genomes. In particular, ORF coding potential is estimated by searching for enrichment in motifs of the X circular code, that is known to be over-represented in the reading frames of viral genes. RESULTS: We applied GOFIX to study the SARS-CoV-2 and related genomes including SARS-CoV and SARS-like viruses from bat, civet and pangolin hosts, focusing on the accessory proteins. Our analysis provides evidence supporting the presence of overlapping ORFs 7b, 9b and 9c in all the genomes and thus helps to resolve some differences in current genome annotations. In contrast, we predict that ORF3b is not functional in all genomes. Novel putative ORFs were also predicted, including a truncated form of the ORF10 previously identified in SARS-CoV-2 and a little known ORF overlapping the Spike protein in Civet-CoV and SARS-CoV. CONCLUSIONS: Our findings contribute to characterizing sequence properties of accessory genes of SARS coronaviruses, and especially the newly acquired genes making use of overlapping reading frames. |*Genome, Viral[MESH] |*Open Reading Frames[MESH] |Animals[MESH] |Betacoronavirus/*genetics[MESH] |Codon[MESH] |Computational Biology[MESH] |Evolution, Molecular[MESH] |Genes, Viral[MESH] |Humans[MESH] |SARS-CoV-2[MESH] |Severe acute respiratory syndrome-related coronavirus/*genetics[MESH] |Spike Glycoprotein, Coronavirus/chemistry/genetics[MESH] |Viral Matrix Proteins/genetics[MESH] |Viral Proteins/chemistry/genetics[MESH]Characterization of accessory genes in coronavirus genomes (epmc).pdf DeepDyve Pubget Overpricing