Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1371/journal.ppat.1008705 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1008705 32853291!7451577!32853291     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Pathog 2020 ; 16 (8): e1008705 Nephropedia Template TP {{tp|p=32853291|t=2020. Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19 |pdf=|usr=}}{{32853291}} gab.com Text Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19 JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=32853291 #FOAvip The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus. Twit Text FOAVip Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19 ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=32853291 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32853291 #FOAvip English Wikipedia <ref>{{Cite pmid|32853291}}</ref> Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19 #MMPMID32853291 Hou T; Zeng W; Yang M; Chen W; Ren L; Ai J; Wu J; Liao Y; Gou X; Li Y; Wang X; Su H; Gu B; Wang J; Xu T PLoS Pathog 2020[Aug]; 16 (8): e1008705 PMID32853291show ga The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus. |*Clinical Laboratory Techniques[MESH] |Bacteria/genetics[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |CRISPR-Cas Systems/*genetics[MESH] |Clustered Regularly Interspaced Short Palindromic Repeats/genetics[MESH] |Coronavirus Infections/*diagnosis/*genetics[MESH] |Genes, Viral/genetics[MESH] |Genome, Viral/genetics[MESH] |High-Throughput Nucleotide Sequencing/methods[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/economics/methods[MESH] |Nucleic Acid Amplification Techniques/economics/methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/*genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction/methods[MESH] |SARS-CoV-2[MESH]Development and evaluation of a rapid CRISPR-based diagnostic for COVI(epmc).pdf DeepDyve Pubget Overpricing