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10.3390/cells9091951 http://scihub22266oqcxt.onion/10.3390/cells9091951 32847031!7563522!32847031     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cells 2020 ; 9 (9): ä Nephropedia Template TP {{tp|p=32847031|t=2020. A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbial Stimuli in Human Cells Could It Play a Role in the Modulation of SARS-CoV-2 Infection?|pdf=|usr=}}{{32847031}} gab.com Text A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbial Stimuli in Human Cells Could It Play a Role in the Modulation of SARS-CoV-2 Infection JO: Cells http://www.kidney.de/pget.php?&tw=1&pmid=32847031 #FOAvip Following influenza infection, rs2248374-G ERAP2 expressing cells may transcribe an alternative spliced isoform: ERAP2/Iso3. This variant, unlike ERAP2-wt, is unable to trim peptides to be loaded on MHC class I molecules, but it can still dimerize with both ERAP2-wt and ERAP1-wt, thus contributing to profiling an alternative cellular immune-peptidome. In order to verify if the expression of ERAP2/Iso3 may be induced by other pathogens, PBMCs and MDMs isolated from 20 healthy subjects were stimulated with flu, LPS, CMV, HIV-AT-2, SARS-CoV-2 antigens to analyze its mRNA and protein expression. In parallel, Calu3 cell lines and PBMCs were in vitro infected with growing doses of SARS-CoV-2 (0.5, 5, 1000 MOI) and HIV-1(BAL) (0.1, 1, and 10 ng p24 HIV-1(Bal)/1 x 10(6) PBMCs) viruses, respectively. Results showed that: (1) ERAP2/Iso3 mRNA expression can be prompted by many pathogens and it is coupled with the modulation of several determinants (cytokines, interferon-stimulated genes, activation/inhibition markers, antigen-presentation elements) orchestrating the anti-microbial immune response (Quantigene); (2) ERAP2/Iso3 mRNA is translated into a protein (western blot); (3) ERAP2/Iso3 mRNA expression is sensitive to SARS-CoV-2 and HIV-1 concentration. Considering the key role played by ERAPs in antigen processing and presentation, it is conceivable that these enzymes may be potential targets and modulators of the pathogenicity of infectious diseases and further analyses are needed to define the role played by the different isoforms. Twit Text FOAVip A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbial Stimuli in Human Cells Could It Play a Role in the Modulation of SARS-CoV-2 Infection ????Cells http://www.kidney.de/pget.php?&tw=1&pmid=32847031 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32847031 #FOAvip English Wikipedia <ref>{{Cite pmid|32847031}}</ref> A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbial Stimuli in Human Cells Could It Play a Role in the Modulation of SARS-CoV-2 Infection? #MMPMID32847031 Saulle I; Vanetti C; Goglia S; Vicentini C; Tombetti E; Garziano M; Clerici M; Biasin M Cells 2020[Aug]; 9 (9): ä PMID32847031show ga Following influenza infection, rs2248374-G ERAP2 expressing cells may transcribe an alternative spliced isoform: ERAP2/Iso3. This variant, unlike ERAP2-wt, is unable to trim peptides to be loaded on MHC class I molecules, but it can still dimerize with both ERAP2-wt and ERAP1-wt, thus contributing to profiling an alternative cellular immune-peptidome. In order to verify if the expression of ERAP2/Iso3 may be induced by other pathogens, PBMCs and MDMs isolated from 20 healthy subjects were stimulated with flu, LPS, CMV, HIV-AT-2, SARS-CoV-2 antigens to analyze its mRNA and protein expression. In parallel, Calu3 cell lines and PBMCs were in vitro infected with growing doses of SARS-CoV-2 (0.5, 5, 1000 MOI) and HIV-1(BAL) (0.1, 1, and 10 ng p24 HIV-1(Bal)/1 x 10(6) PBMCs) viruses, respectively. Results showed that: (1) ERAP2/Iso3 mRNA expression can be prompted by many pathogens and it is coupled with the modulation of several determinants (cytokines, interferon-stimulated genes, activation/inhibition markers, antigen-presentation elements) orchestrating the anti-microbial immune response (Quantigene); (2) ERAP2/Iso3 mRNA is translated into a protein (western blot); (3) ERAP2/Iso3 mRNA expression is sensitive to SARS-CoV-2 and HIV-1 concentration. Considering the key role played by ERAPs in antigen processing and presentation, it is conceivable that these enzymes may be potential targets and modulators of the pathogenicity of infectious diseases and further analyses are needed to define the role played by the different isoforms. |Aminopeptidases/*genetics[MESH] |Antigen Presentation/genetics[MESH] |Betacoronavirus/*immunology[MESH] |Blood Donors[MESH] |COVID-19[MESH] |Cell Line, Tumor[MESH] |Coronavirus Infections/*genetics/virology[MESH] |Gene Expression/immunology[MESH] |Genotype[MESH] |HIV Infections/genetics/virology[MESH] |HIV-1/immunology[MESH] |Humans[MESH] |Immunization/*methods[MESH] |Leukocytes, Mononuclear/metabolism/*virology[MESH] |Macrophages/metabolism/*virology[MESH] |Minor Histocompatibility Antigens/genetics[MESH] |Pandemics[MESH] |Pneumonia, Viral/*genetics/virology[MESH] |Polymorphism, Single Nucleotide[MESH] |Protein Isoforms/*genetics[MESH] |RNA, Messenger/genetics/metabolism[MESH] |SARS-CoV-2[MESH]A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbia(epmc).pdf DeepDyve Pubget Overpricing