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10.1016/j.jviromet.2020.113960 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2020.113960 32835738!7442555!32835738     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2020 ; 285 (ä): 113960 Nephropedia Template TP {{tp|p=32835738|t=2020. A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit |pdf=|usr=}}{{32835738}} gab.com Text A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32835738 #FOAvip The World Health Organization (WHO) has declared a pandemic caused by a new coronavirus named SARS-CoV-2. The growing demand for commercial kits used for automated extraction of SARS-CoV-2 RNA, a key step before rRT-PCR diagnosis, could cause a shortage of stocks that hinders the rapid processing of samples. Although the recommendation is to use automated methods for nucleic acid extraction, alternatives are necessary to replace commercial kits. However, these alternatives should be as reliable as automated methods. This work describes a simple method to detect SARS-CoV-2 from specimens collected in different preservation media. Samples were previously inactivated by heating and precipitating with a PEG/NaCl solution before rRT-PCR assays for Orf1ab, N and S genes. The new method was compared with an automated protocol of nucleic acid extraction. Both procedures showed similar analytical results. Consequently, this simple and inexpensive method is a suitable procedure for laboratory diagnosis of SARS-CoV-2 infection. Twit Text FOAVip A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=32835738 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32835738 #FOAvip English Wikipedia <ref>{{Cite pmid|32835738}}</ref> A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit #MMPMID32835738 Ulloa S; Bravo C; Parra B; Ramirez E; Acevedo A; Fasce R; Fernandez J J Virol Methods 2020[Nov]; 285 (ä): 113960 PMID32835738show ga The World Health Organization (WHO) has declared a pandemic caused by a new coronavirus named SARS-CoV-2. The growing demand for commercial kits used for automated extraction of SARS-CoV-2 RNA, a key step before rRT-PCR diagnosis, could cause a shortage of stocks that hinders the rapid processing of samples. Although the recommendation is to use automated methods for nucleic acid extraction, alternatives are necessary to replace commercial kits. However, these alternatives should be as reliable as automated methods. This work describes a simple method to detect SARS-CoV-2 from specimens collected in different preservation media. Samples were previously inactivated by heating and precipitating with a PEG/NaCl solution before rRT-PCR assays for Orf1ab, N and S genes. The new method was compared with an automated protocol of nucleic acid extraction. Both procedures showed similar analytical results. Consequently, this simple and inexpensive method is a suitable procedure for laboratory diagnosis of SARS-CoV-2 infection. |*Clinical Laboratory Techniques/methods/standards[MESH] |*Real-Time Polymerase Chain Reaction/methods[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |Genes, Viral[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/*virology[MESH] |RNA, Viral/genetics/isolation & purification[MESH]A simple method for SARS-CoV-2 detection by rRT-PCR without the use of(epmc).pdf DeepDyve Pubget Overpricing