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10.1007/978-1-0716-0900-2_17

http://scihub22266oqcxt.onion/10.1007/978-1-0716-0900-2_17
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32833216!ä!32833216

suck abstract from ncbi

pmid32833216      Methods+Mol+Biol 2020 ; 2203 (ä): 231-238
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  • Ribopuromycylation in Coronavirus-Infected Cells #MMPMID32833216
  • Brownsword MJ; Maier HJ; Locker N
  • Methods Mol Biol 2020[]; 2203 (ä): 231-238 PMID32833216show ga
  • Ribopuromycylation enables the visualization and quantitation of translation on a cellular level by immunofluorescence or in total using standard western blotting. This technique uses ribosome catalyzed puromycylation of nascent chains followed by immobilization on the ribosome by antibiotic chain elongation inhibitor emetine. Detection of puromycylated ribosome-bound nascent chains can then be achieved using a puromycin-specific antibody.
  • |Coronavirus Infections[MESH]
  • |Coronavirus/*genetics[MESH]
  • |Fluorescent Antibody Technique[MESH]
  • |Host-Pathogen Interactions[MESH]
  • |Humans[MESH]
  • |Protein Biosynthesis[MESH]
  • |Puromycin/*pharmacology[MESH]


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