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10.1007/978-1-0716-0900-2_14 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0900-2_14 32833213!ä!32833213 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\32833213.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2020 ; 2203 (ä): 187-204 Nephropedia Template TP {{tp|p=32833213|t=2020. Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions |pdf=|usr=}}{{32833213}} gab.com Text Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=32833213 #FOAvip Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., proteins located within a close perimeter of the RTC, can be determined by different proximity labeling approaches using BirA(R118G) (BioID), TurboID, and APEX2. These factors represent a molecular signature of coronavirus RTCs and likely contribute to the viral life cycle, thereby constituting attractive targets for the development of antiviral intervention strategies. Twit Text FOAVip Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=32833213 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32833213 #FOAvip English Wikipedia <ref>{{Cite pmid|32833213}}</ref> Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions #MMPMID32833213 V'kovski P; Steiner S; Thiel V Methods Mol Biol 2020[]; 2203 (ä): 187-204 PMID32833213show ga Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., proteins located within a close perimeter of the RTC, can be determined by different proximity labeling approaches using BirA(R118G) (BioID), TurboID, and APEX2. These factors represent a molecular signature of coronavirus RTCs and likely contribute to the viral life cycle, thereby constituting attractive targets for the development of antiviral intervention strategies. |Animals[MESH] |Ascorbate Peroxidases/genetics[MESH] |Biotinylation[MESH] |Carbon-Nitrogen Ligases/genetics[MESH] |Cell Line[MESH] |Coronavirus/genetics/*pathogenicity[MESH] |Enzymes/*genetics/metabolism[MESH] |Escherichia coli Proteins/genetics[MESH] |Fluorescent Antibody Technique[MESH] |Host-Pathogen Interactions/*physiology[MESH] |Microorganisms, Genetically-Modified[MESH] |Proteomics/*methods[MESH] |Repressor Proteins/genetics[MESH]Proximity Labeling for the Identification of Coronavirus-Host Protein (epmc).pdf DeepDyve Pubget Overpricing