Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText.
Kick-your-searchterm to multiple Engines kick-your-query now !>
A dictionary by aggregated review articles of nephrology, medicine and the life sciences
Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

suck abstract from ncbi


10.1007/978-1-0716-0900-2_14

http://scihub22266oqcxt.onion/10.1007/978-1-0716-0900-2_14
suck pdf from google scholar
32833213!ä!32833213

suck abstract from ncbi


Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
pmid32833213      Methods+Mol+Biol 2020 ; 2203 (ä): 187-204
Nephropedia Template TP

gab.com Text

Twit Text FOAVip

Twit Text #

English Wikipedia


  • Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions #MMPMID32833213
  • V'kovski P; Steiner S; Thiel V
  • Methods Mol Biol 2020[]; 2203 (ä): 187-204 PMID32833213show ga
  • Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., proteins located within a close perimeter of the RTC, can be determined by different proximity labeling approaches using BirA(R118G) (BioID), TurboID, and APEX2. These factors represent a molecular signature of coronavirus RTCs and likely contribute to the viral life cycle, thereby constituting attractive targets for the development of antiviral intervention strategies.
  • |Animals[MESH]
  • |Ascorbate Peroxidases/genetics[MESH]
  • |Biotinylation[MESH]
  • |Carbon-Nitrogen Ligases/genetics[MESH]
  • |Cell Line[MESH]
  • |Coronavirus/genetics/*pathogenicity[MESH]
  • |Enzymes/*genetics/metabolism[MESH]
  • |Escherichia coli Proteins/genetics[MESH]
  • |Fluorescent Antibody Technique[MESH]
  • |Host-Pathogen Interactions/*physiology[MESH]
  • |Microorganisms, Genetically-Modified[MESH]
  • |Proteomics/*methods[MESH]
  • |Repressor Proteins/genetics[MESH]


  • DeepDyve
  • Pubget Overpricing
  • suck abstract from ncbi

    Linkout box