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10.1007/978-1-0716-0900-2_12 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0900-2_12 32833211!ä!32833211 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2020 ; 2203 (ä): 147-165 Nephropedia Template TP {{tp|p=32833211|t=2020. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses |pdf=|usr=}}{{32833211}} gab.com Text Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=32833211 #FOAvip We have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) in which a full-length cDNA corresponding to the IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. Here, we describe the use of transient dominant selection as a method for introducing modifications into the IBV cDNA that has been successfully used for the substitution of specific nucleotides, deletion of genomic regions, and the exchange of complete genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA-dependent RNA polymerase. Twit Text FOAVip Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=32833211 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32833211 #FOAvip English Wikipedia <ref>{{Cite pmid|32833211}}</ref> Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses #MMPMID32833211 Keep S; Britton P; Bickerton E Methods Mol Biol 2020[]; 2203 (ä): 147-165 PMID32833211show ga We have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) in which a full-length cDNA corresponding to the IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. Here, we describe the use of transient dominant selection as a method for introducing modifications into the IBV cDNA that has been successfully used for the substitution of specific nucleotides, deletion of genomic regions, and the exchange of complete genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA-dependent RNA polymerase. |Animals[MESH] |Bacteriophages/genetics[MESH] |Chlorocebus aethiops[MESH] |DNA-Directed DNA Polymerase/metabolism[MESH] |Fowlpox virus/genetics[MESH] |Homologous Recombination[MESH] |Infectious bronchitis virus/*genetics[MESH] |Microorganisms, Genetically-Modified[MESH] |Transfection/*methods[MESH] |Vaccinia virus/*genetics/isolation & purification[MESH]Transient Dominant Selection for the Modification and Generation of Re(epmc).pdf DeepDyve Pubget Overpricing