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Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Methods+Mol+Biol 2020 ; 2203 (ä): 135-143 Nephropedia Template TP
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Quantification of Coronaviruses by Titration In Vitro and Ex Vivo #MMPMID32833210
Dowgier G; Maier HJ
Methods Mol Biol 2020[]; 2203 (ä): 135-143 PMID32833210show ga
Several techniques are currently available to quickly and accurately quantify the number of virus particles in a sample, taking advantage of advanced technologies improving old techniques or generating new ones, generally relying on partial detection methods or structural analysis. Therefore, characterization of virus infectivity in a sample is often essential, and classical virological methods are extremely powerful in providing accurate results even in an old-fashioned way. In this chapter, we describe in detail the techniques routinely used to estimate the number of viable infectious coronavirus particles in a given sample. All these techniques are serial dilution assays, also known as titrations or end-point dilution assays (EPDA).