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10.1007/978-1-0716-0900-2_11

http://scihub22266oqcxt.onion/10.1007/978-1-0716-0900-2_11
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32833210!ä!32833210

suck abstract from ncbi


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pmid32833210      Methods+Mol+Biol 2020 ; 2203 (ä): 135-143
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  • Quantification of Coronaviruses by Titration In Vitro and Ex Vivo #MMPMID32833210
  • Dowgier G; Maier HJ
  • Methods Mol Biol 2020[]; 2203 (ä): 135-143 PMID32833210show ga
  • Several techniques are currently available to quickly and accurately quantify the number of virus particles in a sample, taking advantage of advanced technologies improving old techniques or generating new ones, generally relying on partial detection methods or structural analysis. Therefore, characterization of virus infectivity in a sample is often essential, and classical virological methods are extremely powerful in providing accurate results even in an old-fashioned way. In this chapter, we describe in detail the techniques routinely used to estimate the number of viable infectious coronavirus particles in a given sample. All these techniques are serial dilution assays, also known as titrations or end-point dilution assays (EPDA).
  • |Animals[MESH]
  • |Cells, Cultured[MESH]
  • |Coronavirus/growth & development/*pathogenicity[MESH]
  • |Infectious bronchitis virus/growth & development/pathogenicity[MESH]
  • |Trachea/cytology[MESH]


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