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10.1007/978-1-0716-0900-2_10

http://scihub22266oqcxt.onion/10.1007/978-1-0716-0900-2_10
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32833209!ä!32833209

suck abstract from ncbi


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pmid32833209      Methods+Mol+Biol 2020 ; 2203 (ä): 119-134
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  • Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures #MMPMID32833209
  • Gultom M; Laloli L; Dijkman R
  • Methods Mol Biol 2020[]; 2203 (ä): 119-134 PMID32833209show ga
  • Well-differentiated primary airway epithelial cell (AEC) cultures have been widely used for the characterization of several human respiratory viruses including coronaviruses. In recent years, there has been an increase in interest toward animal AEC cultures and their application to characterize veterinary viruses with zoonotic potential, as well as studying host-pathogen interactions in animal reservoir host species. In this chapter, we provide a revised and improved protocol for the isolation and establishment of well-differentiated AEC cultures from diverse mammalian species and the use of the cultures for the characterization of veterinary coronavirus. We also describe immunohistochemistry protocols with validated antibodies for the visualization and identification of viral cell tropism in well-differentiated AEC cultures from human, swine, bovine, and feline origin.
  • |Animals[MESH]
  • |Bronchi/*cytology[MESH]
  • |Cats[MESH]
  • |Cattle[MESH]
  • |Cell Differentiation[MESH]
  • |Coronavirus/*physiology[MESH]
  • |Epithelial Cells/cytology/*virology[MESH]
  • |Fluorescent Antibody Technique[MESH]
  • |Humans[MESH]
  • |Immunohistochemistry/methods[MESH]
  • |Primary Cell Culture/instrumentation/*methods[MESH]
  • |Swine[MESH]
  • |Trachea/*cytology[MESH]


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