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Detection and Discovery of Coronaviruses in Wild Bird Populations #MMPMID32833202
Snoeck CJ; Zohari S
Methods Mol Biol 2020[]; 2203 (ä): 41-53 PMID32833202show ga
Wild birds are natural hosts of multiple microbial agents, including a wide diversity of coronaviruses. Here we describe a pan-Coronavirus detection RT-PCR method to identify those viruses regardless of the coronavirus genus or nature of the specimen. We also describe a protocol using high-throughput sequencing technologies to obtain their entire genome, which overcomes the inherent difficulties of wild bird coronavirus sequencing, that is, their genetic diversity and the lack of virus isolation methods.