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10.1007/978-1-0716-0900-2_3

http://scihub22266oqcxt.onion/10.1007/978-1-0716-0900-2_3
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32833202!ä!32833202

suck abstract from ncbi


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pmid32833202      Methods+Mol+Biol 2020 ; 2203 (ä): 41-53
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  • Detection and Discovery of Coronaviruses in Wild Bird Populations #MMPMID32833202
  • Snoeck CJ; Zohari S
  • Methods Mol Biol 2020[]; 2203 (ä): 41-53 PMID32833202show ga
  • Wild birds are natural hosts of multiple microbial agents, including a wide diversity of coronaviruses. Here we describe a pan-Coronavirus detection RT-PCR method to identify those viruses regardless of the coronavirus genus or nature of the specimen. We also describe a protocol using high-throughput sequencing technologies to obtain their entire genome, which overcomes the inherent difficulties of wild bird coronavirus sequencing, that is, their genetic diversity and the lack of virus isolation methods.
  • |Animals[MESH]
  • |Animals, Wild[MESH]
  • |Bird Diseases/*virology[MESH]
  • |Coronavirus Infections/genetics/*veterinary[MESH]
  • |Coronavirus/*genetics/*isolation & purification[MESH]
  • |RNA-Dependent RNA Polymerase/genetics[MESH]
  • |Real-Time Polymerase Chain Reaction/*methods[MESH]


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