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10.3390/s20164648

http://scihub22266oqcxt.onion/10.3390/s20164648
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32824787!7472328!32824787
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suck abstract from ncbi


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pmid32824787      Sensors+(Basel) 2020 ; 20 (16): ä
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  • DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment #MMPMID32824787
  • Santhanam M; Algov I; Alfonta L
  • Sensors (Basel) 2020[Aug]; 20 (16): ä PMID32824787show ga
  • Pandemics require a fast and immediate response to contain potential infectious carriers. In the recent 2020 Covid-19 worldwide pandemic, authorities all around the world have failed to identify potential carriers and contain it on time. Hence, a rapid and very sensitive testing method is required. Current diagnostic tools, reverse transcription PCR (RT-PCR) and real-time PCR (qPCR), have its pitfalls for quick pandemic containment such as the requirement for specialized professionals and instrumentation. Versatile electrochemical DNA/RNA sensors are a promising technological alternative for PCR based diagnosis. In an electrochemical DNA sensor, a nucleic acid hybridization event is converted into a quantifiable electrochemical signal. A critical challenge of electrochemical DNA sensors is sensitive detection of a low copy number of DNA/RNA in samples such as is the case for early onset of a disease. Signal amplification approaches are an important tool to overcome this sensitivity issue. In this review, the authors discuss the most recent signal amplification strategies employed in the electrochemical DNA/RNA diagnosis of pathogens.
  • |*Biosensing Techniques[MESH]
  • |*Electrochemical Techniques[MESH]
  • |Betacoronavirus/*isolation & purification/pathogenicity[MESH]
  • |COVID-19[MESH]
  • |Coronavirus Infections/*diagnosis/epidemiology/virology[MESH]
  • |DNA/isolation & purification[MESH]
  • |Epidemics/prevention & control[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*diagnosis/epidemiology/virology[MESH]
  • |RNA/isolation & purification[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]


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