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10.1016/j.jcv.2020.104593

http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104593
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32823131!7429071!32823131
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suck abstract from ncbi


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pmid32823131      J+Clin+Virol 2020 ; 131 (ä): 104593
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  • Evaluation on testing of deep throat saliva and lower respiratory tract specimens with Xpert Xpress SARS-CoV-2 assay #MMPMID32823131
  • Wong RC; Wong AH; Ho YI; Leung EC; Lai RW
  • J Clin Virol 2020[Oct]; 131 (ä): 104593 PMID32823131show ga
  • BACKGROUND: Xpert(R) Xpress SARS-CoV-2 assay is only validated on nasopharyngeal specimens for detection of SARS-CoV-2. Other specimen types such as deep throat saliva (DTS), also known as posterior oropharyngeal saliva and lower-respiratorytract specimens (LRT) including sputum, tracheal aspirate and bronchoalveolar lavage are not validated. These non-validated specimen types, however, do have significant diagnostic value. OBJECTIVE: Evaluate the performance of Xpert Xpress SARS-CoV-2 assay for detection of SARS-CoV-2 from DTS and LRT specimens. METHODS: 162 specimens from 158 patients with suspected COVID-19 disease were tested with Xpert Xpress SARS-CoV-2 assay. These included 120 DTS and 42 LRT specimens i.e. 35 sputum, 6 tracheal aspirate and one bronchoalveolar lavage. Results were compared to those by the TIB-Molbiol LightMix(R) SarbecoV E-gene assay. RESULTS: Xpert Xpress SARS-CoV-2 assay has satisfactory performance when compared with reference method. The positive percent agreement (PPA) of DTS and LRT specimens were 98.86 % & 100 % respectively while the negative percent agreement (NPA) was 100 % for both DTS and LRT specimens. CONCLUSIONS: This study demonstrated with appropriate sample pre-treatment, Xpert Xpress SARS-CoV-2 assay can be used to test on non-validated specimen types including DTS & LRT specimens.
  • |*Clinical Laboratory Techniques[MESH]
  • |Adult[MESH]
  • |Betacoronavirus[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Coronavirus Infections/*diagnosis[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Molecular Diagnostic Techniques[MESH]
  • |Nasopharynx/virology[MESH]
  • |Oropharynx/virology[MESH]
  • |Pandemics[MESH]
  • |Pharynx/virology[MESH]
  • |Pneumonia, Viral/*diagnosis[MESH]
  • |Reagent Kits, Diagnostic[MESH]
  • |Respiratory System/*virology[MESH]
  • |SARS-CoV-2[MESH]
  • |Saliva/*virology[MESH]
  • |Sensitivity and Specificity[MESH]


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