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10.1016/j.virusres.2020.198129 http://scihub22266oqcxt.onion/10.1016/j.virusres.2020.198129 32822689!7434412!32822689     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virus+Res 2020 ; 288 (ä): 198129 Nephropedia Template TP {{tp|p=32822689|t=2020. iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2 |pdf=|usr=}}{{32822689}} gab.com Text iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2 JO: Virus Res http://www.kidney.de/pget.php?&tw=1&pmid=32822689 #FOAvip The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are urgently needed to manage and control the spread of SARS-CoV-2. RT-qPCR based methods are the gold standard for SARS-CoV-2 detection. However, these methods require trained personnel, sophisticated infrastructure, and a long turnaround time, thereby limiting their usefulness. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP), a one-step nucleic acid amplification method conducted at a single temperature, has been used for colorimetric virus detection. CRISPR-Cas12 and CRISPR-Cas13 systems, which possess collateral activity against ssDNA and RNA, respectively, have also been harnessed for virus detection. Here, we built an efficient, rapid, specific, sensitive, user-friendly SARS-CoV-2 detection module that combines the robust virus amplification of RT-LAMP with the specific detection ability of SARS-CoV-2 by CRISPR-Cas12. Furthermore, we combined the RT-LAMP-CRISPR-Cas12 module with lateral flow cells to enable highly efficient point-of-care SARS-CoV-2 detection. Our iSCAN SARS-CoV-2 detection module, which exhibits the critical features of a robust molecular diagnostic device, should facilitate the effective management and control of COVID-19. Twit Text FOAVip iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2 ????Virus Res http://www.kidney.de/pget.php?&tw=1&pmid=32822689 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32822689 #FOAvip English Wikipedia <ref>{{Cite pmid|32822689}}</ref> iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2 #MMPMID32822689 Ali Z; Aman R; Mahas A; Rao GS; Tehseen M; Marsic T; Salunke R; Subudhi AK; Hala SM; Hamdan SM; Pain A; Alofi FS; Alsomali A; Hashem AM; Khogeer A; Almontashiri NAM; Abedalthagafi M; Hassan N; Mahfouz MM Virus Res 2020[Oct]; 288 (ä): 198129 PMID32822689show ga The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly are urgently needed to manage and control the spread of SARS-CoV-2. RT-qPCR based methods are the gold standard for SARS-CoV-2 detection. However, these methods require trained personnel, sophisticated infrastructure, and a long turnaround time, thereby limiting their usefulness. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP), a one-step nucleic acid amplification method conducted at a single temperature, has been used for colorimetric virus detection. CRISPR-Cas12 and CRISPR-Cas13 systems, which possess collateral activity against ssDNA and RNA, respectively, have also been harnessed for virus detection. Here, we built an efficient, rapid, specific, sensitive, user-friendly SARS-CoV-2 detection module that combines the robust virus amplification of RT-LAMP with the specific detection ability of SARS-CoV-2 by CRISPR-Cas12. Furthermore, we combined the RT-LAMP-CRISPR-Cas12 module with lateral flow cells to enable highly efficient point-of-care SARS-CoV-2 detection. Our iSCAN SARS-CoV-2 detection module, which exhibits the critical features of a robust molecular diagnostic device, should facilitate the effective management and control of COVID-19. |*CRISPR-Cas Systems[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/instrumentation/*methods[MESH] |Colorimetry/instrumentation/*methods[MESH] |Coronavirus Infections/*diagnosis/virology[MESH] |Endodeoxyribonucleases/chemistry[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/instrumentation/*methods[MESH] |Nucleic Acid Amplification Techniques/instrumentation/*methods[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |Point-of-Care Systems[MESH] |Rheology[MESH] |SARS-CoV-2[MESH]iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive det(epmc).pdf DeepDyve Pubget Overpricing