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10.1080/22221751.2020.1813636 http://scihub22266oqcxt.onion/10.1080/22221751.2020.1813636 32819220!8284965!32819220     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Emerg+Microbes+Infect 2020 ; 9 (1): 1965-1973 Nephropedia Template TP {{tp|p=32819220|t=2020. Accurate serology for SARS-CoV-2 and common human coronaviruses using a multiplex approach |pdf=|usr=}}{{32819220}} gab.com Text Accurate serology for SARS-CoV-2 and common human coronaviruses using a multiplex approach JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32819220 #FOAvip Serology is a crucial part of the public health response to the ongoing SARS-CoV-2 pandemic. Here, we describe the development, validation and clinical evaluation of a protein micro-array as a quantitative multiplex immunoassay that can identify S and N-directed SARS-CoV-2 IgG antibodies with high specificity and sensitivity and distinguish them from all currently circulating human coronaviruses. The method specificity was 100% for SARS-CoV-2 S1 and 96% for N antigen based on extensive syndromic (n=230 cases) and population panel (n=94) testing that also confirmed the high prevalence of seasonal human coronaviruses. To assess its potential role for both SARS-CoV-2 patient diagnostics and population studies, we evaluated a large heterogeneous COVID-19 cohort (n=330) and found an overall sensitivity of 89% (>/= 21 days post onset symptoms (dps)), ranging from 86% to 96% depending on severity of disease. For a subset of these patients longitudinal samples were provided up to 56 dps. Mild cases showed absent or delayed, and lower SARS-CoV-2 antibody responses. Overall, we present the development and extensive clinical validation of a multiplex coronavirus serological assay for syndromic testing, to answer research questions regarding to antibody responses, to support SARS-CoV-2 diagnostics and to evaluate epidemiological developments efficiently and with high-throughput. Twit Text FOAVip Accurate serology for SARS-CoV-2 and common human coronaviruses using a multiplex approach ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32819220 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32819220 #FOAvip English Wikipedia <ref>{{Cite pmid|32819220}}</ref> Accurate serology for SARS-CoV-2 and common human coronaviruses using a multiplex approach #MMPMID32819220 van Tol S; Mogling R; Li W; Godeke GJ; Swart A; Bergmans B; Brandenburg A; Kremer K; Murk JL; van Beek J; Wintermans B; Reimerink J; Bosch BJ; Reusken C Emerg Microbes Infect 2020[Dec]; 9 (1): 1965-1973 PMID32819220show ga Serology is a crucial part of the public health response to the ongoing SARS-CoV-2 pandemic. Here, we describe the development, validation and clinical evaluation of a protein micro-array as a quantitative multiplex immunoassay that can identify S and N-directed SARS-CoV-2 IgG antibodies with high specificity and sensitivity and distinguish them from all currently circulating human coronaviruses. The method specificity was 100% for SARS-CoV-2 S1 and 96% for N antigen based on extensive syndromic (n=230 cases) and population panel (n=94) testing that also confirmed the high prevalence of seasonal human coronaviruses. To assess its potential role for both SARS-CoV-2 patient diagnostics and population studies, we evaluated a large heterogeneous COVID-19 cohort (n=330) and found an overall sensitivity of 89% (>/= 21 days post onset symptoms (dps)), ranging from 86% to 96% depending on severity of disease. For a subset of these patients longitudinal samples were provided up to 56 dps. Mild cases showed absent or delayed, and lower SARS-CoV-2 antibody responses. Overall, we present the development and extensive clinical validation of a multiplex coronavirus serological assay for syndromic testing, to answer research questions regarding to antibody responses, to support SARS-CoV-2 diagnostics and to evaluate epidemiological developments efficiently and with high-throughput. |Aged[MESH] |Antibodies, Viral/*blood[MESH] |Antigens, Viral/blood/immunology[MESH] |Betacoronavirus/*immunology/pathogenicity[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods/standards[MESH] |Coronavirus Infections/*diagnosis/immunology/mortality/virology[MESH] |Coronavirus Nucleocapsid Proteins[MESH] |Female[MESH] |Humans[MESH] |Longitudinal Studies[MESH] |Male[MESH] |Middle Aged[MESH] |Middle East Respiratory Syndrome Coronavirus/immunology/pathogenicity[MESH] |Neutralization Tests[MESH] |Nucleocapsid Proteins/*blood/immunology[MESH] |Pandemics[MESH] |Phosphoproteins[MESH] |Pneumonia, Viral/*diagnosis/immunology/mortality/virology[MESH] |Protein Array Analysis[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Severe acute respiratory syndrome-related coronavirus/immunology/pathogenicity[MESH] |Severity of Illness Index[MESH]Accurate serology for SARS-CoV-2 and common human coronaviruses using (epmc).pdf DeepDyve Pubget Overpricing