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10.1126/scitranslmed.abc3103 http://scihub22266oqcxt.onion/10.1126/scitranslmed.abc3103 32817357!7665313!32817357     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Transl+Med 2020 ; 12 (559): ä Nephropedia Template TP {{tp|p=32817357|t=2020. A comparison of four serological assays for detecting anti-SARS-CoV-2 antibodies in human serum samples from different populations |pdf=|usr=}}{{32817357}} gab.com Text A comparison of four serological assays for detecting anti-SARS-CoV-2 antibodies in human serum samples from different populations JO: Sci Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=32817357 #FOAvip It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their differing antibody response profiles. Here, we performed a pilot study of four serological assays to assess the amounts of anti-SARS-CoV-2 antibodies in serum samples obtained from 491 healthy individuals before the SARS-CoV-2 pandemic, 51 individuals hospitalized with COVID-19, 209 suspected cases of COVID-19 with mild symptoms, and 200 healthy blood donors. We used two ELISA assays that recognized the full-length nucleoprotein (N) or trimeric spike (S) protein ectodomain of SARS-CoV-2. In addition, we developed the S-Flow assay that recognized the S protein expressed at the cell surface using flow cytometry, and the luciferase immunoprecipitation system (LIPS) assay that recognized diverse SARS-CoV-2 antigens including the S1 domain and the carboxyl-terminal domain of N by immunoprecipitation. We obtained similar results with the four serological assays. Differences in sensitivity were attributed to the technique and the antigen used. High anti-SARS-CoV-2 antibody titers were associated with neutralization activity, which was assessed using infectious SARS-CoV-2 or lentiviral-S pseudotype virus. In hospitalized patients with COVID-19, seroconversion and virus neutralization occurred between 5 and 14 days after symptom onset, confirming previous studies. Seropositivity was detected in 32% of mildly symptomatic individuals within 15 days of symptom onset and in 3% of healthy blood donors. The four antibody assays that we used enabled a broad evaluation of SARS-CoV-2 seroprevalence and antibody profiling in different subpopulations within one region. Twit Text FOAVip A comparison of four serological assays for detecting anti-SARS-CoV-2 antibodies in human serum samples from different populations ????Sci Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=32817357 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32817357 #FOAvip English Wikipedia <ref>{{Cite pmid|32817357}}</ref> A comparison of four serological assays for detecting anti-SARS-CoV-2 antibodies in human serum samples from different populations #MMPMID32817357 Grzelak L; Temmam S; Planchais C; Demeret C; Tondeur L; Huon C; Guivel-Benhassine F; Staropoli I; Chazal M; Dufloo J; Planas D; Buchrieser J; Rajah MM; Robinot R; Porrot F; Albert M; Chen KY; Crescenzo-Chaigne B; Donati F; Anna F; Souque P; Gransagne M; Bellalou J; Nowakowski M; Backovic M; Bouadma L; Le Fevre L; Le Hingrat Q; Descamps D; Pourbaix A; Laouenan C; Ghosn J; Yazdanpanah Y; Besombes C; Jolly N; Pellerin-Fernandes S; Cheny O; Ungeheuer MN; Mellon G; Morel P; Rolland S; Rey FA; Behillil S; Enouf V; Lemaitre A; Creach MA; Petres S; Escriou N; Charneau P; Fontanet A; Hoen B; Bruel T; Eloit M; Mouquet H; Schwartz O; van der Werf S Sci Transl Med 2020[Sep]; 12 (559): ä PMID32817357show ga It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their differing antibody response profiles. Here, we performed a pilot study of four serological assays to assess the amounts of anti-SARS-CoV-2 antibodies in serum samples obtained from 491 healthy individuals before the SARS-CoV-2 pandemic, 51 individuals hospitalized with COVID-19, 209 suspected cases of COVID-19 with mild symptoms, and 200 healthy blood donors. We used two ELISA assays that recognized the full-length nucleoprotein (N) or trimeric spike (S) protein ectodomain of SARS-CoV-2. In addition, we developed the S-Flow assay that recognized the S protein expressed at the cell surface using flow cytometry, and the luciferase immunoprecipitation system (LIPS) assay that recognized diverse SARS-CoV-2 antigens including the S1 domain and the carboxyl-terminal domain of N by immunoprecipitation. We obtained similar results with the four serological assays. Differences in sensitivity were attributed to the technique and the antigen used. High anti-SARS-CoV-2 antibody titers were associated with neutralization activity, which was assessed using infectious SARS-CoV-2 or lentiviral-S pseudotype virus. In hospitalized patients with COVID-19, seroconversion and virus neutralization occurred between 5 and 14 days after symptom onset, confirming previous studies. Seropositivity was detected in 32% of mildly symptomatic individuals within 15 days of symptom onset and in 3% of healthy blood donors. The four antibody assays that we used enabled a broad evaluation of SARS-CoV-2 seroprevalence and antibody profiling in different subpopulations within one region. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Antibodies, Viral/*blood[MESH] |Betacoronavirus/*immunology[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Cohort Studies[MESH] |Coronavirus Infections/*diagnosis/epidemiology/immunology[MESH] |Enzyme-Linked Immunosorbent Assay/methods[MESH] |Female[MESH] |Flow Cytometry/methods[MESH] |France/epidemiology[MESH] |Healthy Volunteers[MESH] |Humans[MESH] |Immunoprecipitation/methods[MESH] |Luciferases[MESH] |Male[MESH] |Middle Aged[MESH] |Neutralization Tests[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/epidemiology/immunology[MESH] |SARS-CoV-2[MESH] |Seroepidemiologic Studies[MESH] |Serologic Tests/*methods[MESH] |Spike Glycoprotein, Coronavirus/immunology[MESH] |Translational Research, Biomedical[MESH]A comparison of four serological assays for detecting anti-SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing