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10.2144/btn-2020-0057

http://scihub22266oqcxt.onion/10.2144/btn-2020-0057
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32815744!7439763!32815744
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suck abstract from ncbi

pmid32815744      Biotechniques 2020 ; 69 (4): 317-325
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  • PCR past, present and future #MMPMID32815744
  • Zhu H; Zhang H; Xu Y; Lassakova S; Korabecna M; Neuzil P
  • Biotechniques 2020[Oct]; 69 (4): 317-325 PMID32815744show ga
  • PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.
  • |Animals[MESH]
  • |Betacoronavirus/genetics/isolation & purification[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/instrumentation/methods[MESH]
  • |Coronavirus Infections/diagnosis/virology[MESH]
  • |Equipment Design[MESH]
  • |Humans[MESH]
  • |Microfluidic Analytical Techniques/instrumentation/methods[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/diagnosis/virology[MESH]
  • |Point-of-Care Systems[MESH]
  • |Polymerase Chain Reaction/instrumentation/*methods[MESH]


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