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10.1002/2211-5463.12958 http://scihub22266oqcxt.onion/10.1002/2211-5463.12958 32810913!7530398!32810913     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       FEBS+Open+Bio 2020 ; 10 (10): 2166-2181 Nephropedia Template TP {{tp|p=32810913|t=2020. Secreted cystatins decrease proliferation and enhance apoptosis of human leukemic cells |pdf=|usr=}}{{32810913}} gab.com Text Secreted cystatins decrease proliferation and enhance apoptosis of human leukemic cells JO: FEBS Open Bio http://www.kidney.de/pget.php?&tw=1&pmid=32810913 #FOAvip Cysteine proteases are implicated in proteolysis events favoring cancer cell growth, spread, and death by apoptosis. Herein, we have studied whether the net growth and survival of the leukemic cell lines Jurkat, U937, and HL-60 are affected by external addition of five proteins acting as natural cysteine protease inhibitors. None of the cystatins examined (A, C, D, and E/M) or chagasin showed consistent effects on Fas-induced apoptosis when evaluated at 1 microm. In contrast, when the intrinsic apoptosis pathway was activated by hydrogen peroxide, addition of cystatin D augmented caspase-3-like activity within all three cell lines. Flow cytometric analysis of U937 cells also showed increased numbers of annexin V-positive cells when hydrogen peroxide was used to initiate apoptosis and cells were cultured in the presence of cystatin D or C. Moreover, stimulation of hydrogen peroxide-induced apoptotic U937 cells with either cystatin C or D resulted in a dose-dependent decrease in the number of cells. Cell viability was also decreased when U937 cells were cultured in the presence of cystatin C or D (1-9 microm) only, demonstrating that these cystatins can reduce cell proliferation by themselves in addition to enhancing apoptosis induced by oxidative stress. These effects on U937 cells were paralleled by internalization of cystatins C and D, indicating these effects are caused by downregulation of intracellular proteolysis. External addition of cystatins C and D to HL-60 and Jurkat cells demonstrated similar degrees of cystatin D uptake and decreased viability as for U937 cells, indicating that these effects are general for leukemic cells. Twit Text FOAVip Secreted cystatins decrease proliferation and enhance apoptosis of human leukemic cells ????FEBS Open Bio http://www.kidney.de/pget.php?&tw=1&pmid=32810913 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32810913 #FOAvip English Wikipedia <ref>{{Cite pmid|32810913}}</ref> Secreted cystatins decrease proliferation and enhance apoptosis of human leukemic cells #MMPMID32810913 Hunaiti S; Wallin H; Eriksson M; Jaras M; Abrahamson M FEBS Open Bio 2020[Oct]; 10 (10): 2166-2181 PMID32810913show ga Cysteine proteases are implicated in proteolysis events favoring cancer cell growth, spread, and death by apoptosis. Herein, we have studied whether the net growth and survival of the leukemic cell lines Jurkat, U937, and HL-60 are affected by external addition of five proteins acting as natural cysteine protease inhibitors. None of the cystatins examined (A, C, D, and E/M) or chagasin showed consistent effects on Fas-induced apoptosis when evaluated at 1 microm. In contrast, when the intrinsic apoptosis pathway was activated by hydrogen peroxide, addition of cystatin D augmented caspase-3-like activity within all three cell lines. Flow cytometric analysis of U937 cells also showed increased numbers of annexin V-positive cells when hydrogen peroxide was used to initiate apoptosis and cells were cultured in the presence of cystatin D or C. Moreover, stimulation of hydrogen peroxide-induced apoptotic U937 cells with either cystatin C or D resulted in a dose-dependent decrease in the number of cells. Cell viability was also decreased when U937 cells were cultured in the presence of cystatin C or D (1-9 microm) only, demonstrating that these cystatins can reduce cell proliferation by themselves in addition to enhancing apoptosis induced by oxidative stress. These effects on U937 cells were paralleled by internalization of cystatins C and D, indicating these effects are caused by downregulation of intracellular proteolysis. External addition of cystatins C and D to HL-60 and Jurkat cells demonstrated similar degrees of cystatin D uptake and decreased viability as for U937 cells, indicating that these effects are general for leukemic cells. |Apoptosis/drug effects[MESH] |Cell Death/drug effects[MESH] |Cell Line, Tumor/drug effects[MESH] |Cell Proliferation/drug effects[MESH] |Cell Survival/drug effects[MESH] |Cystatin C/metabolism/*pharmacology[MESH] |Cystatins/metabolism/*pharmacology/physiology[MESH] |Cysteine Proteinase Inhibitors/pharmacology[MESH] |Humans[MESH] |Leukemia/genetics/*metabolism[MESH] |Proteolysis[MESH]Secreted cystatins decrease proliferation and enhance apoptosis of hum(epmc).pdf DeepDyve Pubget Overpricing