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Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Eur+J+Clin+Microbiol+Infect+Dis 2020 ; 39 (12): 2289-2297 Nephropedia Template TP
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Evaluation of three immunochromatographic tests for rapid detection of antibodies against SARS-CoV-2 #MMPMID32808111
Lateral flow immunoassays (LFIA) for rapid detection of specific antibodies (IgM and IgG) against SARS-CoV-2 in different human specimens have been developed in response to the pandemic. The aim of this study is to evaluate three immunocromathographic assays (Sienna(R), Wondfo(R) and Prometheus(R)) for detection of antibodies against SARS-CoV-2 in serum samples, considering RT-qPCR as a reference. A total of 145 serum samples from 145 patients with clinical suspicion of COVID-19 were collected: all of the samples were tested with Sienna(R), 117 with Wondfo(R) and 89 with Prometheus(R). The overall results of sensitivity, specificity, positive predictive value and negative predictive value obtained were as follows: 64.4%, 75%, 85.5% and 47.8% with Sienna(R); 45.2%, 81.8%, 80.5% and 47.4% with Wondfo(R) and 75.5%, 12.5%, 51.4% and 29.4% with Prometheus(R). The accuracy of the test for Sienna(R), Wondfo(R) and Prometheus(R) was 67.6%, 59% and 47.2%, with a prevalence of COVID-19 of 69.7%, 62.4% and 55.1% respectively. Sensitivity of the three tests (Sienna(R), Wondfo(R) and Prometheus(R) respectively) along the three different stages was 36.6%, 18.8% and 68.6% in the early stage (first week); 81.3%, 74.1% and 90.9% in the intermediate stage (second week) and 100%, 83.3% and 100% in the late stage (third week). The results demonstrate that even though Prometheus(R) presented a high sensitivity, the specificity was notably lower than the other two tests. Sienna(R) showed the greatest contrast between sensitivity and specificity, achieving the best accuracy, followed by Wondfo(R). The sensitivity of the three ICT assays was higher in late stages of the disease.