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Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays #MMPMID32798217
Patterson EI; Prince T; Anderson ER; Casas-Sanchez A; Smith SL; Cansado-Utrilla C; Solomon T; Griffiths MJ; Acosta-Serrano A; Turtle L; Hughes GL
J Infect Dis 2020[Oct]; 222 (9): 1462-1467 PMID32798217show ga
The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80 degrees C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels.
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J+Infect+Dis 2020 ; 222 (9): 1462-1467
