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10.1016/j.ijid.2020.08.015 http://scihub22266oqcxt.onion/10.1016/j.ijid.2020.08.015 32791207!7416699!32791207     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Infect+Dis 2020 ; 99 (ä): 352-354 Nephropedia Template TP {{tp|p=32791207|t=2020. Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction |pdf=|usr=}}{{32791207}} gab.com Text Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction JO: Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32791207 #FOAvip The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction, by comparing the efficacy of RT-qPCR in several direct approaches versus the gold standard RNA extraction, in the detection of SARS-CoV-2 in laboratory samples, as well as in clinical oro-nasopharyngeal SARS-CoV-2 swabs. The findings showed an advantage for the extraction procedure; however a direct no-buffer approach might be an alternative, since it identified more than 60% of positive clinical specimens. Twit Text FOAVip Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction ????Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32791207 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32791207 #FOAvip English Wikipedia <ref>{{Cite pmid|32791207}}</ref> Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction #MMPMID32791207 Israeli O; Beth-Din A; Paran N; Stein D; Lazar S; Weiss S; Milrot E; Atiya-Nasagi Y; Yitzhaki S; Laskar O; Schuster O Int J Infect Dis 2020[Oct]; 99 (ä): 352-354 PMID32791207show ga The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction, by comparing the efficacy of RT-qPCR in several direct approaches versus the gold standard RNA extraction, in the detection of SARS-CoV-2 in laboratory samples, as well as in clinical oro-nasopharyngeal SARS-CoV-2 swabs. The findings showed an advantage for the extraction procedure; however a direct no-buffer approach might be an alternative, since it identified more than 60% of positive clinical specimens. |Animals[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*diagnosis[MESH] |Feasibility Studies[MESH] |Humans[MESH] |Nasal Cavity/virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |RNA, Viral/genetics/*isolation & purification[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2[MESH]Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in com(epmc).pdf DeepDyve Pubget Overpricing