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10.1016/j.jcv.2020.104572 http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104572 32769024!7396134!32769024     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32769024&cmd=llinks): Failed to open stream: HTTP request failed! 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Evaluation of a novel multiplexed assay for determining IgG levels and functional activity to SARS-CoV-2 |pdf=|usr=}}{{32769024}} gab.com Text Evaluation of a novel multiplexed assay for determining IgG levels and functional activity to SARS-CoV-2 JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32769024 #FOAvip BACKGROUND: The emergence of SARS-CoV-2 has led to the development of serological assays that could aid in an understanding of the burden of COVID-19 disease. Many available tests lack rigorous evaluation and therefore results may be misleading. OBJECTIVES: The aim of this study was to assess the performance of a novel multiplexed immunoassay for the simultaneous detection of antibodies against SARS-CoV-2 trimeric spike (S), spike receptor binding domain (RBD), spike N terminal domain and nucleocapsid antigen and a novel pseudo-neutralisation assay. METHODS: A multiplexed solid-phase chemiluminescence assay (Meso Scale Discovery) was evaluated for the simultaneous detection of IgG binding to four SARS-CoV-2 antigens and the quantification of antibody-induced ACE-2 binding inhibition (pseudo-neutralisation assay). Sensitivity was evaluated with a total of 196 COVID-19 serum samples (169 confirmed PCR positive and 27 anti-nucleocapsid IgG positive) from individuals with mild symptomatic or asymptomatic disease. Specificity was evaluated with 194 control serum samples collected from adults prior to December 2019. RESULTS: The specificity and sensitivity of the binding IgG assay was highest for S protein with a specificity of 97.4 % and sensitivity of 96.2 % for samples taken 14 days and 97.9 % for samples taken 21 days following the onset of symptoms. IgG concentration to S and RBD correlated strongly with percentage inhibition measured by the pseudo-neutralisation assay. CONCLUSION: Excellent sensitivity for IgG detection was obtained over 14 days since onset of symptoms for three SARS-CoV-2 antigens (S, RBD and N) in this multiplexed assay which can also measure antibody functionality. Twit Text FOAVip Evaluation of a novel multiplexed assay for determining IgG levels and functional activity to SARS-CoV-2 ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32769024 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32769024 #FOAvip English Wikipedia <ref>{{Cite pmid|32769024}}</ref> Evaluation of a novel multiplexed assay for determining IgG levels and functional activity to SARS-CoV-2 #MMPMID32769024 Johnson M; Wagstaffe HR; Gilmour KC; Mai AL; Lewis J; Hunt A; Sirr J; Bengt C; Grandjean L; Goldblatt D J Clin Virol 2020[Sep]; 130 (ä): 104572 PMID32769024show ga BACKGROUND: The emergence of SARS-CoV-2 has led to the development of serological assays that could aid in an understanding of the burden of COVID-19 disease. Many available tests lack rigorous evaluation and therefore results may be misleading. OBJECTIVES: The aim of this study was to assess the performance of a novel multiplexed immunoassay for the simultaneous detection of antibodies against SARS-CoV-2 trimeric spike (S), spike receptor binding domain (RBD), spike N terminal domain and nucleocapsid antigen and a novel pseudo-neutralisation assay. METHODS: A multiplexed solid-phase chemiluminescence assay (Meso Scale Discovery) was evaluated for the simultaneous detection of IgG binding to four SARS-CoV-2 antigens and the quantification of antibody-induced ACE-2 binding inhibition (pseudo-neutralisation assay). Sensitivity was evaluated with a total of 196 COVID-19 serum samples (169 confirmed PCR positive and 27 anti-nucleocapsid IgG positive) from individuals with mild symptomatic or asymptomatic disease. Specificity was evaluated with 194 control serum samples collected from adults prior to December 2019. RESULTS: The specificity and sensitivity of the binding IgG assay was highest for S protein with a specificity of 97.4 % and sensitivity of 96.2 % for samples taken 14 days and 97.9 % for samples taken 21 days following the onset of symptoms. IgG concentration to S and RBD correlated strongly with percentage inhibition measured by the pseudo-neutralisation assay. CONCLUSION: Excellent sensitivity for IgG detection was obtained over 14 days since onset of symptoms for three SARS-CoV-2 antigens (S, RBD and N) in this multiplexed assay which can also measure antibody functionality. |Adult[MESH] |Antibodies, Viral/*blood[MESH] |Antigens, Viral/*immunology[MESH] |Betacoronavirus[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques[MESH] |Coronavirus Infections/*diagnosis/immunology[MESH] |Coronavirus Nucleocapsid Proteins[MESH] |Female[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Immunoglobulin G/*blood[MESH] |Luminescent Measurements/methods[MESH] |Male[MESH] |Middle Aged[MESH] |Nucleocapsid Proteins/immunology[MESH] |Pandemics[MESH] |Phosphoproteins[MESH] |Pneumonia, Viral/*diagnosis/immunology[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH]Evaluation of a novel multiplexed assay for determining IgG levels and(epmc).pdf DeepDyve Pubget Overpricing